Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway

Su,Liping; Qiao,Qiao; Li,Ruifeng; Wu,Huiguang

doi:10.3892/etm.2018.5948

Leptin attenuates the growth of rabbit mesenchymal stem cells via the extracellular signal-regulated kinase signaling pathway

Authors:
- Liping Su
- Qiao Qiao
- Ruifeng Li
- Huiguang Wu
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Affiliations: School of Basic Medical Science, Inner Mongolia Medical University, Hohhot, Inner Mongolia 010010, P.R. China, Obstetrics and Gynecology Department, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010010, P.R. China, Department of Cervical Surgery, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010010, P.R. China, Animal Science College, Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia 028000, P.R. China
Published online on: March 12, 2018 https://doi.org/10.3892/etm.2018.5948
Pages: 4185-4190
Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

When stimulated, mesenchymal stem cells (MSCs) may differentiate into chondroblasts, adipocytes or osteoblasts. Leptin is an adipocyte‑derived hormone, which regulates food intake and glucose homeostasis. The aim of the present study was to identify the potential role of mitogen‑activated protein kinase in the leptin‑induced growth of rabbit bone MSCs (rBMSCs). Various concentrations of leptin were used to culture rBMSCs and the viability of cells was observed as well as alterations in the phosphorylation state of extracellular signal‑regulated kinase 1/2 (ERK1/2), c‑Jun N‑terminal kinase and p38. It was revealed that the growth of leptin‑treated rBMSCs was primarily inhibited by phosphorylated ERK1/2, which was mediated by the leptin receptor. In conclusion, the results of the present study demonstrated that leptin inhibits the growth of rBMSCs principally via the ERK1/2 signaling pathway.

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1	Zhang Y and Wang W: Effects of bone marrow mesenchymal stem cell transplantation on light-damaged retina. Invest Ophthalmol Vis Sci. 51:3742–3748. 2010. View Article : Google Scholar : PubMed/NCBI
2	Zheng H, Martin JA, Duwayri Y, Falcon G and Buckwalter JA: Impact of aging on rat bone marrow-derived stem cell chondrogenesis. J Gerontol A Biol Sci Med Sci. 62:136–148. 2007. View Article : Google Scholar : PubMed/NCBI
3	van Marion AM, Thiele J, Kvasnicka HM and van den Tweel JG: Morphology of the bone marrow after stem cell transplantation. Histopathology. 48:329–342. 2006. View Article : Google Scholar : PubMed/NCBI
4	Xu B, Zhang J, Brewer E, Tu Q, Yu L, Tang J, Krebsbach P, Wieland M and Chen J: Osterix enhances BMSC-associated osseointegration of implants. J Dent Res. 88:1003–1007. 2009. View Article : Google Scholar : PubMed/NCBI
5	Zheng Q, Banaszak L, Fracci S, Basali D, Dunlap SM, Hursting SD, Rich JN, Hjlemeland AB, Vasanji A, Berger NA, et al: Leptin receptor maintains cancer stem-like properties in triple negative breast cancer cells. Endocr Relat Cancer. 20:797–808. 2013. View Article : Google Scholar : PubMed/NCBI
6	Zheng B, Jiang J, Luo K, Liu L, Lin M, Chen Y and Yan F: Increased osteogenesis in osteoporotic bone marrow stromal cells by overexpression of leptin. Cell Tissue Res. 36:845–856. 2015. View Article : Google Scholar
7	Tse HF, Yiu KH and Lau CP: Bone marrow stem cell therapy for myocardial angiogenesis. Curr Vasc Pharmacol. 5:103–112. 2007. View Article : Google Scholar : PubMed/NCBI
8	Noda T, Kikugawa T, Tanji N, Miura N, Asai S, Higashiyama S and Yokoyama M: Long-term exposure to leptin enhances the growth of prostate cancer cells. Int J Oncol. 46:1535–1542. 2015. View Article : Google Scholar : PubMed/NCBI
9	Saucillo DC, Gerriets VA, Sheng J, Rathmell JC and Maciver NJ: Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol. 192:136–144. 2014. View Article : Google Scholar : PubMed/NCBI
10	Kurtovic S, Ng TT, Gupta A, Arumugaswami V, Chaiboonma KL, Aminzadeh MA, Makkar R, Dafoe DC and Talavera-Adame D: Leptin enhances endothelial cell differentiation and angiogenesis in murine embryonic stem cells. Microvasc Res. 97:65–74. 2015. View Article : Google Scholar : PubMed/NCBI
11	Wang WZ, Yao XD, Huang XJ, Li JQ and Xu H: Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage. Exp Ther Med. 10:995–1002. 2015. View Article : Google Scholar : PubMed/NCBI
12	Nokhbehsaim M, Keser S, Nogueira AV, Jäger A, Jepsen S, Cirelli JA, Bourauel C, Eick S and Deschner J: Leptin effects on the regenerative capacity of human periodontal cells. Int J Endocrinol. 2014:1803042014. View Article : Google Scholar : PubMed/NCBI
13	Cassano S, Pucino V, La Rocca C, Procaccini C, De Rosa V, Marone G and Matarese G: Leptin modulates autophagy in human CD4+CD25-conventional T cells. Metabolism. 63:1272–1279. 2014. View Article : Google Scholar : PubMed/NCBI
14	Reis BS, Lee K, Fanok MH, Mascaraque C, Amoury M, Cohn LB, Rogoz A, Dallner OS, Moraes-Vieira PM, Domingos AI and Mucida D: Leptin receptor signaling in T cells is required for Th17 differentiation. J Immunol. 194:5253–5260. 2015. View Article : Google Scholar : PubMed/NCBI
15	Dupuis L, Schuermann Y, Cohen T, Siddappa D, Kalaiselvanraja A, Pansera M, Bordignon V and Duggavathi R: Role of leptin receptors in granulosa cells during ovulation. Reproduction. 147:221–229. 2013. View Article : Google Scholar : PubMed/NCBI
16	Réus GZ, Vieira FG, Abelaira HM, Michels M, Tomaz DB, dos Santos MA, Carlessi AS, Neotti MV, Matias BI, Luz JR, et al: MAPK signaling correlates with the antidepressant effects of ketamine. J Psychiatr Res. 55:15–21. 2014. View Article : Google Scholar : PubMed/NCBI
17	Delhanty PJ, van der Eerden BC, van der Velde M, Gauna C, Pols HA, Jahr H, Chiba H, van der Lely AJ and van Leeuwen JP: Ghrelin and unacylated ghrelin stimulate human osteoblast growth via mitogen-activated protein kinase (MAPK)/phosphoinositide 3-kinase (PI3K) pathways in the absence of GHS-R1a. J Endocrinol. 188:37–47. 2006. View Article : Google Scholar : PubMed/NCBI