Open Access

Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance

  • Authors:
    • Jing Tong
    • Xi‑Jiang Hu
    • Wen‑Qian Cai
    • Xiang Dai
    • Lei Wang
  • View Affiliations

  • Published online on: March 22, 2018     https://doi.org/10.3892/etm.2018.5990
  • Pages: 4441-4447
  • Copyright: © Tong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Puerarin has long been used as a traditional Chinese medicine, which possesses various physiological properties, including anti‑inflammation, anti‑oxidative, anti‑diabetic and anti‑cancer activities. The aim of the current study was to investigate the effect of puerarin on delayed‑type hypersensitivity (DTH) induced by ovalbumin (OVA) in mice and explore its underlying mechanisms. The results showed that puerarin significantly attenuated DTH, resulting from a decrement in footpad swelling, reduction in inflammatory cell as well as a decline in anti‑OVA IgG in serum. In the homogenized supernatant of footpad tissues, the classic Th1‑cytokines, including interferon (IFN)‑γ was suppressed following puerarin treatment. Furthermore, a high dose of puerarin inhibited interleukin (IL)‑4 production, the classic Th2‑cytokine. The concanavalin A stimulation and MTT assays indicated a suppressive effect of puerarin on Th1 response via decreasing IFN‑γ production in OVA‑primed lymphocytes. Detailed studies revealed that puerarin modulated the Th1/Th2 balance in DTH responses, attributing to lower T‑bet/GATA binding protein‑3 mRNA and protein level ratios, which led to the shift change of IFN‑γ/IL‑4 with puerarin treatment. These findings demonstrate that puerarin alleviated inflammation in DTH triggered by OVA application via curbing inflammatory cytokines by modulating the Th1/Th2 balance. These results suggest that puerarin may be an alternative therapeutic option for the treatment of DTH.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tong J, Hu XJ, Cai WQ, Dai X and Wang L: Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance. Exp Ther Med 15: 4441-4447, 2018.
APA
Tong, J., Hu, X., Cai, W., Dai, X., & Wang, L. (2018). Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance. Experimental and Therapeutic Medicine, 15, 4441-4447. https://doi.org/10.3892/etm.2018.5990
MLA
Tong, J., Hu, X., Cai, W., Dai, X., Wang, L."Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance". Experimental and Therapeutic Medicine 15.5 (2018): 4441-4447.
Chicago
Tong, J., Hu, X., Cai, W., Dai, X., Wang, L."Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4441-4447. https://doi.org/10.3892/etm.2018.5990