Open Access

Oral immunization with rotavirus VP7‑CTB fusion expressed in transgenic Arabidopsis thaliana induces antigen‑specific IgA and IgG and passive protection in mice

  • Authors:
    • Yuxian Li
    • Lili Guan
    • Xiuming Liu
    • Weican Liu
    • Jing Yang
    • Xiaomei Zhang
    • Fawei Wang
    • Yongxin Guo
    • Haiyan Li
    • Xiaokun Li
  • View Affiliations

  • Published online on: March 28, 2018     https://doi.org/10.3892/etm.2018.6003
  • Pages: 4866-4874
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Human rotavirus (HRV) is the primary cause of severe gastroenteritis in children. However, there is currently no protective virus for rotavirus available. In the present study, an HRVVP7‑cholera toxin B subunit (CTB) fusion protein was expressed in Arabidopsis thaliana. To determine the adjuvant effect of HRVVP7‑CTB, HRVVP7 without CTB was expressed in the same manner. HRVVP7‑CTB accounted for 0.39% of the total soluble protein (TSP) in the transgenic seeds and 52.65 µg/g of HRVVP7 protein was expressed in these seeds. Mice were immunized with TSP from the transformed seeds and produced serum immunoglobulin G (IgG) and mucosal IgA specifically directed against HRVVP7. Antibody titers were highest in mice orally immunized with the plant‑expressed HRVVP7‑CTB protein, whereas HRVVP7‑CTB‑specific IgG neutralized the rotavirus. Suckling pups born from dams immunized with the HRVVP7‑CTB fusion protein were protected against challenge with virulent rotavirus. The results of the present study suggest that the HRVVP7‑CTB fusion protein produced in A. thaliana may be a rotaviral‑specific candidate subunit vaccine.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li Y, Guan L, Liu X, Liu W, Yang J, Zhang X, Wang F, Guo Y, Li H, Li X, Li X, et al: Oral immunization with rotavirus VP7‑CTB fusion expressed in transgenic Arabidopsis thaliana induces antigen‑specific IgA and IgG and passive protection in mice. Exp Ther Med 15: 4866-4874, 2018
APA
Li, Y., Guan, L., Liu, X., Liu, W., Yang, J., Zhang, X. ... Li, X. (2018). Oral immunization with rotavirus VP7‑CTB fusion expressed in transgenic Arabidopsis thaliana induces antigen‑specific IgA and IgG and passive protection in mice. Experimental and Therapeutic Medicine, 15, 4866-4874. https://doi.org/10.3892/etm.2018.6003
MLA
Li, Y., Guan, L., Liu, X., Liu, W., Yang, J., Zhang, X., Wang, F., Guo, Y., Li, H., Li, X."Oral immunization with rotavirus VP7‑CTB fusion expressed in transgenic Arabidopsis thaliana induces antigen‑specific IgA and IgG and passive protection in mice". Experimental and Therapeutic Medicine 15.6 (2018): 4866-4874.
Chicago
Li, Y., Guan, L., Liu, X., Liu, W., Yang, J., Zhang, X., Wang, F., Guo, Y., Li, H., Li, X."Oral immunization with rotavirus VP7‑CTB fusion expressed in transgenic Arabidopsis thaliana induces antigen‑specific IgA and IgG and passive protection in mice". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4866-4874. https://doi.org/10.3892/etm.2018.6003