Effect of miRNA-19a on gastrointestinal motility in rats with functional dyspepsia

Deng,Yinzhi; Zhou,Xiangyu; Xiang,Xingchao; Ou,Yangli; He,Jianhua

doi:10.3892/etm.2018.6009

Effect of miRNA-19a on gastrointestinal motility in rats with functional dyspepsia

Authors:
- Yinzhi Deng
- Xiangyu Zhou
- Xingchao Xiang
- Yangli Ou
- Jianhua He
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Affiliations: Department of Gastroenterology, The Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China
Published online on: March 30, 2018 https://doi.org/10.3892/etm.2018.6009
Pages: 4875-4879
Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effect of microRNA (miRNA)-19a on gastrointestinal motility in rats with functional dyspepsia was investigated. Fifty adult Sprague-Dawley (SD) rats were randomly divided into 5 groups, 10 rats in each group, one group as the normal group, one group as the model group, and the other three groups were divided into negative control group, miRNA-19a mimic group and miRNA-19a inhibitor group. All rats were intraperitoneally injected with miRNA‑19a scramble, miRNA-19a mimic and miRNA-19a inhibitor. Except the normal group, the functional dyspepsia model rat was established by proper clipping tail stimulation. The gastric emptying rate, intestinal propulsive ratio, serum motilin and vasoactive intestinal peptide of rats in each group were measured. The level of miRNA-19a expression in each group was detected by reverse transcription-polymerase chain reaction (RT-PCR). The gastric emptying rate, intestinal propulsive ratio and serum motilin in model group were significantly lower than those in normal group, and vasoactive intestinal peptide was higher in model group than that in normal group (P<0.05). The expression of miRNA-19a in model group was significantly higher than that in normal group (P<0.05). After intraperitoneal injection of miRNA-19a mimic, the expression of miRNA-19a was increased; gastric emptying rate, intestinal propulsive ratio and serum motilin were significantly reduced in model group, and vasoactive intestinal peptide was increased (P<0.05). After intraperitoneal injection of miRNA-19a inhibitor, the expression of miRNA-19a was remarkably decreased; gastric emptying rate, intestinal propulsive ratio and serum motilin were further increased in model group, and vasoactive intestinal peptide was decreased (P<0.05). In conclusion, the expression of miRNA-19a in rats with functional dyspepsia is higher than that in normal rats, and the reduced miRNA-19a expression can ameliorate the gastrointestinal motility in rats with functional dyspepsia.

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1	Imthon AK, Moeller ME, Drewes AM, Drewes AM, Juel J and Aziz Q: Functional gastroduodenal disorders. Hamdan Med J. 8:253–263. 2015.
2	Ghoshal UC, Singh R, Chang FY, Hou X, Wong BC and Kachintorn U: Functional Dyspepsia Consensus Team of the Asian Neurogastroenterology and Motility Association and the Asian Pacific Association of Gastroenterology: Epidemiology of uninvestigated and functional dyspepsia in Asia: Facts and fiction. J Neurogastroenterol Motil. 17:235–244. 2011. View Article : Google Scholar : PubMed/NCBI
3	Zhang GS, Xie S, Hu W, Liu RE, Liu ML, Liu M and Chang XY: Effects of electroacupuncture on interstitial cells of Cajal (ICC) ultrastructure and connexin 43 protein expression in the gastrointestinal tract of functional dyspepsia (FD) rats. Med Sci Monit. 22:2021–2027. 2016. View Article : Google Scholar : PubMed/NCBI
4	El-Serag HB and Talley NJ: Systemic review: The prevalence and clinical course of functional dyspepsia. Aliment Pharmacol Ther. 19:643–654. 2004. View Article : Google Scholar : PubMed/NCBI
5	He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI
6	Wang D, Fan Z, Liu F and Zuo J: Hsa-miR-21 and Hsa-miR-29 in tissue as potential diagnostic and prognostic biomarkers for gastric cancer. Cell Physiol Biochem. 37:1454–1462. 2015. View Article : Google Scholar : PubMed/NCBI
7	Ueda T, Volinia S, Okumura H, Shimizu M, Taccioli C, Rossi S, Alder H, Liu CG, Oue N, Yasui W, et al: Relation between microRNA expression and progression and prognosis of gastric cancer: A microRNA expression analysis. Lancet Oncol. 11:136–146. 2010. View Article : Google Scholar : PubMed/NCBI
8	Saito Y, Suzuki H, Imaeda H, Matsuzaki J, Hirata K, Tsugawa H, Hibino S, Kanai Y, Saito H and Hibi T: The tumor suppressor microRNA-29c is downregulated and restored by celecoxib in human gastric cancer cells. Int J Cancer. 132:1751–1760. 2013. View Article : Google Scholar : PubMed/NCBI
9	Kapeller J, Houghton LA, Mönnikes H, Walstab J, Möller D, Bönisch H, Burwinkel B, Autschbach F, Funke B, Lasitschka F, et al: First evidence for an association of a functional variant in the microRNA-510 target site of the serotonin receptor-type 3E gene with diarrhea predominant irritable bowel syndrome. Hum Mol Genet. 17:2967–2977. 2008. View Article : Google Scholar : PubMed/NCBI
10	Zhou Q, Souba WW, Croce CM and Verne GN: MicroRNA-29a regulates intestinal membrane permeability in patients with irritable bowel syndrome. Gut. 59:775–784. 2010. View Article : Google Scholar : PubMed/NCBI
11	Saito Y, Suzuki H, Tsugawa H, Suzuki S, Matsuzaki J, Hirata K and Hibi T: Dysfunctional gastric emptying with down-regulation of muscle-specific microRNAs in Helicobacter pylori-infected mice. Gastroenterology. 140:189–198. 2011. View Article : Google Scholar : PubMed/NCBI
12	Abdel-Aziz H, Wadie W, Zaki HF, Müller J, Kelber O, Efferth T and Khayyal MT: Novel sequential stress model for functional dyspepsia: Efficacy of the herbal preparation STW5. Phytomedicine. 22:588–595. 2015. View Article : Google Scholar : PubMed/NCBI
13	Talley NJ: Functional dyspepsia: New insights into pathogenesis and therapy. Korean J Intern Med. 31:444–456. 2016. View Article : Google Scholar : PubMed/NCBI
14	Hao M, Zang M, Wendlandt E, Xu Y, An G, Gong D, Li F, Qi F, Zhang Y, Yang Y, et al: Low serum miR-19a expression as a novel poor prognostic indicator in multiple myeloma. Int J Cancer. 136:1835–1844. 2015. View Article : Google Scholar : PubMed/NCBI
15	Feng Y, Liu J, Kang Y, He Y, Liang B, Yang P and Yu Z: miR-19a acts as an oncogenic microRNA and is up-regulated in bladder cancer. J Exp Clin Cancer Res. 33:672014. View Article : Google Scholar : PubMed/NCBI
16	Baik SH, Lee J, Lee YS, Jang JY and Kim CW: ANT2 shRNA downregulates miR-19a and miR-96 through the PI3K/Akt pathway and suppresses tumor growth in hepatocellular carcinoma cells. Exp Mol Med. 48:e2222016. View Article : Google Scholar : PubMed/NCBI
17	Yamamoto K, Ito S, Hanafusa H, Shimizu K and Ouchida M: Uncovering direct targets of miR-19a involved in lung cancer progression. PLoS One. 10:e01378872015. View Article : Google Scholar : PubMed/NCBI
18	Huang L, Wang X, Wen C, Yang X, Song M, Chen J, Wang C, Zhang B, Wang L, Iwamoto A, et al: Hsa-miR-19a is associated with lymph metastasis and mediates the TNF-α induced epithelial-to-mesenchymal transition in colorectal cancer. Sci Rep. 5:133502015. View Article : Google Scholar : PubMed/NCBI
19	Lu W, Xu Z, Zhang M and Zuo Y: MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer. Int J Clin Exp Pathol. 7:7286–7296. 2014.PubMed/NCBI
20	Yang F, Wang H, Jiang Z, Hu A, Chu L, Sun Y and Han J: MicroRNA-19a mediates gastric carcinoma cell proliferation through the activation of nuclear factor-κB. Mol Med Rep. 12:5780–5786. 2015. View Article : Google Scholar : PubMed/NCBI
21	Sanger GJ and Furness JB: Ghrelin and motilin receptors as drug targets for gastrointestinal disorders. Nat Rev Gastroenterol Hepatol. 13:38–48. 2016. View Article : Google Scholar : PubMed/NCBI