Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation

  • Authors:
    • Tong Li
    • Wenqian Zhang
    • Qing Xu
    • Shentao Li
    • Xuehong Tong
    • Jie Ding
    • Hui Li
    • Shengcai Hou
    • Zhidong Xu
    • David M. Jablons
    • Liang You
  • View Affiliations

  • Published online on: April 13, 2018     https://doi.org/10.3892/etm.2018.6058
  • Pages: 4961-4971
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Abstract

Donor organ rejection remains a significant problem. The present study aimed to assess whether transferring a donor's major histocompatibility complex (MHC) genes to the recipient could mitigate rejection in organ transplantation. Seven loci of MHC genes from donor mice were amplified and ligated into vectors; the vectors either contained one K locus, seven loci or were empty (control). The vectors were subsequently injected into the thymus of recipients (in heterotransplants, recipient rats received the vector containing one K locus), following which donor mouse hearts were transplanted. Following the transplantation of allograft and heterograft, electrocardiosignals were viable for a significantly longer duration in recipient mice and rats receiving the donor histocompatibility‑2 complex (H‑2)d genes compared with those in controls, and in mice that received seven vectors compared with those receiving one vector. Mixed lymphocyte cultures containing cells from these recipients proliferated significantly less compared with mixed lymphocyte cultures containing controls. Also, hearts from H‑2d genes‑treated recipients demonstrated less lymphocyte infiltration and necrosis compared with the control recipient. The present study concluded that allograft and heterograft rejection may be mitigated by introducing the donor's MHC into the recipient; transferring seven loci has been demonstrated to be more effective than transferring one locus.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li T, Zhang W, Xu Q, Li S, Tong X, Ding J, Li H, Hou S, Xu Z, Jablons DM, Jablons DM, et al: Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation. Exp Ther Med 15: 4961-4971, 2018.
APA
Li, T., Zhang, W., Xu, Q., Li, S., Tong, X., Ding, J. ... You, L. (2018). Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation. Experimental and Therapeutic Medicine, 15, 4961-4971. https://doi.org/10.3892/etm.2018.6058
MLA
Li, T., Zhang, W., Xu, Q., Li, S., Tong, X., Ding, J., Li, H., Hou, S., Xu, Z., Jablons, D. M., You, L."Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation". Experimental and Therapeutic Medicine 15.6 (2018): 4961-4971.
Chicago
Li, T., Zhang, W., Xu, Q., Li, S., Tong, X., Ding, J., Li, H., Hou, S., Xu, Z., Jablons, D. M., You, L."Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4961-4971. https://doi.org/10.3892/etm.2018.6058