Open Access

Spontaneous remission of obstructive jaundice in rats: Selection of experimental models

  • Authors:
    • Yunfu Lv
    • Jie Yue
    • Xiaoguang Gong
    • Xiaoyu Han
    • Hongfei Wu
    • Jie Deng
    • Yejuan Li
  • View Affiliations

  • Published online on: May 2, 2018     https://doi.org/10.3892/etm.2018.6119
  • Pages: 5295-5301
  • Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to evaluate the prevalence and causes of spontaneous remission of obstructive jaundice in rats. Healthy male and female Wistar rats (180‑220 g) were randomly assigned to receive common bile duct ligation (CBDL) and transection (group A), CBDL only (group B), or CBD dissection without ligation or transection (control group C; n=36 in each group). There was a difference in eye and skin jaundice prevalence between groups A and B from 14 days after surgery. The level of total bilirubin (TB) did not continue to increase in group A and began to decrease in the majority of rats in group B (P<0.05 vs. group B). At day 21 after surgery, the TB level returned to normal in group B and no significant difference was observed compared with group C. At day 21 after surgery, significant dilatation of bile ducts above the ligature was observed in group A following cholangiography with 38% meglumine diatrizoate and this contrast agent did not spread to other sites. Slight dilatation of the proximal bile ducts was observed in group B and the contrast agent entered the intestinal lumen through the omental ducts adhering to the porta hepatis. After 14 days of surgery, there were 36 rats in group A and B, and 17 rats exhibited spontaneous regression of jaundice. Overall, 47.2% (17/36) of rats experienced spontaneous remission of obstructive jaundice, 82.4% (14/17) of which underwent ligation only. The spontaneous remission of jaundice may have been caused by shunting through very small bile ducts or omental ducts adhering to the porta hepatis. If a model of biliary obstruction is to be established in future research, a model of CBDL and transection is preferable. In this case, jaundice reduction surgery should be performed 14 days after establishment of the model.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Lv Y, Yue J, Gong X, Han X, Wu H, Deng J and Li Y: Spontaneous remission of obstructive jaundice in rats: Selection of experimental models. Exp Ther Med 15: 5295-5301, 2018.
APA
Lv, Y., Yue, J., Gong, X., Han, X., Wu, H., Deng, J., & Li, Y. (2018). Spontaneous remission of obstructive jaundice in rats: Selection of experimental models. Experimental and Therapeutic Medicine, 15, 5295-5301. https://doi.org/10.3892/etm.2018.6119
MLA
Lv, Y., Yue, J., Gong, X., Han, X., Wu, H., Deng, J., Li, Y."Spontaneous remission of obstructive jaundice in rats: Selection of experimental models". Experimental and Therapeutic Medicine 15.6 (2018): 5295-5301.
Chicago
Lv, Y., Yue, J., Gong, X., Han, X., Wu, H., Deng, J., Li, Y."Spontaneous remission of obstructive jaundice in rats: Selection of experimental models". Experimental and Therapeutic Medicine 15, no. 6 (2018): 5295-5301. https://doi.org/10.3892/etm.2018.6119