Open Access

Evaluation of blood vessel injury, oxidative stress and circulating inflammatory factors in an L‑NAME‑induced preeclampsia‑like rat model

  • Authors:
    • Wen Shu
    • Hanying Li
    • Hao Gong
    • Mei Zhang
    • Xiulong Niu
    • Yongqiang Ma
    • Xin Zhang
    • Wei Cai
    • Guohong Yang
    • Maoti Wei
    • Ning Yang
    • Yuming Li
  • View Affiliations

  • Published online on: May 24, 2018     https://doi.org/10.3892/etm.2018.6217
  • Pages: 585-594
  • Copyright: © Shu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Preeclampsia is a pregnancy‑specific disease characterized by hypertension as well as proteinuria after the 20th week of pregnancy. Animal models are effective tools for studying the pathogenesis, diagnostic criteria and treatment methods of preeclampsia. The present study sought to establish and evaluate a preeclampsia‑like Sprague Dawley (SD) rat model using N‑nitro‑L‑arginine methyl ester (L‑NAME). Rats were randomly assigned to 7 groups (n=10 in each): Control rats and rats treated with low‑dose L‑NAME (40 mg/kg body weight/day) starting from gestational day (GD) 9, medium‑L‑NAME (75 mg/kg body weight/day) starting from GD 9 (9D ML group), high‑dose L‑NAME (125 mg/kg body weight/day) starting from GD 9, low‑dose L‑NAME starting from GD 10, medium‑dose L‑NAME starting from GD 10 and high‑dose L‑NAME starting from GD 10. Blood pressure (BP), 24‑h proteinuria, fetal intrauterine growth, histopathological changes, the plasma soluble fms‑like tyrosine kinase‑1 (sFlt‑1)/placental growth factor (PLGF) ratio and cytokine levels were evaluated. Elevated BP, increased urinary albumin excretion, severe endotheliosis, mesangial expansion and increased sFlt‑1/PLGF ratios were observed in the experimental groups compared with the control group (P<0.05), particularly in the 9D ML group. The results of the present study may optimize the conditions of the previously established L‑NAME‑induced preeclampsia SD rat model and aid further study into the pathogenesis of preeclampsia.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Shu W, Li H, Gong H, Zhang M, Niu X, Ma Y, Zhang X, Cai W, Yang G, Wei M, Wei M, et al: Evaluation of blood vessel injury, oxidative stress and circulating inflammatory factors in an L‑NAME‑induced preeclampsia‑like rat model. Exp Ther Med 16: 585-594, 2018.
APA
Shu, W., Li, H., Gong, H., Zhang, M., Niu, X., Ma, Y. ... Li, Y. (2018). Evaluation of blood vessel injury, oxidative stress and circulating inflammatory factors in an L‑NAME‑induced preeclampsia‑like rat model. Experimental and Therapeutic Medicine, 16, 585-594. https://doi.org/10.3892/etm.2018.6217
MLA
Shu, W., Li, H., Gong, H., Zhang, M., Niu, X., Ma, Y., Zhang, X., Cai, W., Yang, G., Wei, M., Yang, N., Li, Y."Evaluation of blood vessel injury, oxidative stress and circulating inflammatory factors in an L‑NAME‑induced preeclampsia‑like rat model". Experimental and Therapeutic Medicine 16.2 (2018): 585-594.
Chicago
Shu, W., Li, H., Gong, H., Zhang, M., Niu, X., Ma, Y., Zhang, X., Cai, W., Yang, G., Wei, M., Yang, N., Li, Y."Evaluation of blood vessel injury, oxidative stress and circulating inflammatory factors in an L‑NAME‑induced preeclampsia‑like rat model". Experimental and Therapeutic Medicine 16, no. 2 (2018): 585-594. https://doi.org/10.3892/etm.2018.6217