MicroRNA‑379 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by targeting tumor protein D52 Retraction in /10.3892/etm.2024.12577

Zhao,Xiaojun; Chu,Jiusheng

doi:10.3892/etm.2018.6302

MicroRNA‑379 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by targeting tumor protein D52

Retraction in: /10.3892/etm.2024.12577

Authors:
- Xiaojun Zhao
- Jiusheng Chu
View Affiliations

Affiliations: Department of Otolaryngology and Head Surgery, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China
Published online on: June 13, 2018 https://doi.org/10.3892/etm.2018.6302
Pages: 1232-1240
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

MicroRNAs (miRs) have been demonstrated to be important regulators of malignant behavior in nasopharyngeal carcinoma (NPC) tumorigenesis. The present study aimed to investigate the biological roles and underlying mechanisms of miR‑379 in NPC. The study initially observed that miR‑379 was significantly downregulated in NPC clinical tissues and cell lines using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Next, gain‑of‑function assays were performed on human the NPC cell lines, C666‑1 and 5‑8F, including MTT, colony formation and transwell migration assays. The results indicated that ectopic expression of miR‑379 suppressed the NPC cell proliferation, colony formation, migration and invasion in vitro. In addition, tumor protein D52 (TPD52) was identified as a direct target of miR‑379 by a dual‑luciferase reporter assay, while overexpression of miR‑379 markedly reduced TPD52 expression at the mRNA and protein levels, as determined by RT‑qPCR and western blot analysis, respectively. Furthermore, silencing of TPD52 significantly inhibited the C666‑1 cell proliferation, migration and invasion. These findings suggest that miR‑379 negatively regulates the growth and migration of NPC cells by downregulating TPD52 expression, while modulation of miR‑379 expression may be a therapeutic strategy for NPC.

View Figures

View References

1	Raab-Traub N: Epstein-Barr virus in the pathogenesis of NPC. Semin Cancer Biol. 12:431–441. 2002. View Article : Google Scholar : PubMed/NCBI
2	Breda E, Catarino RJ, Azevedo I, Lobão M, Monteiro E and Medeiros R: Epstein-Barr virus detection in nasopharyngeal carcinoma: Implications in a low-risk area. Braz J Otorhinolaryngol. 76:310–315. 2010. View Article : Google Scholar : PubMed/NCBI
3	Chia WK, Teo M, Wang WW, Lee B, Ang SF, Tai WM, Chee CL, Ng J, Kan R, Lim WT, et al: Adoptive T-cell transfer and chemotherapy in the first-line treatment of metastatic and/or locally recurrent nasopharyngeal carcinoma. Mol Ther. 22:132–139. 2014. View Article : Google Scholar : PubMed/NCBI
4	Kavitha N, Vijayarathna S, Jothy SL, Oon CE, Chen Y, Kanwar JR and Sasidharan S: MicroRNAs: Biogenesis, roles for carcinogenesis and as potential biomarkers for cancer diagnosis and prognosis. Asian Pac J Cancer Prev. 15:7489–7497. 2014. View Article : Google Scholar : PubMed/NCBI
5	Esquela-Kerscher A and Slack FJ: Oncomirs-microRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI
6	Xia H, Ng SS, Jiang S, Cheung WK, Sze J, Bian XW, Kung HF and Lin MC: miR-200a-mediated downregulation of ZEB2 and CTNNB1 differentially inhibits nasopharyngeal carcinoma cell growth, migration and invasion. Biochem Biophys Res Commun. 391:535–541. 2010. View Article : Google Scholar : PubMed/NCBI
7	Zhang LY, Ho-Fun Lee V, Wong AM, Kwong DL, Zhu YH, Dong SS, Kong KL, Chen J, Tsao SW, Guan XY and Fu L: MicroRNA-144 promotes cell proliferation, migration and invasion in nasopharyngeal carcinoma through repression of PTEN. Carcinogenesis. 34:454–463. 2013. View Article : Google Scholar : PubMed/NCBI
8	Wong TS, Man OY, Tsang CM, Tsao SW, Tsang RK, Chan JY, Ho WK, Wei WI and To VS: MicroRNA let-7 suppresses nasopharyngeal carcinoma cells proliferation through downregulating c-Myc expression. J Cancer Res Clin Oncol. 137:415–422. 2011. View Article : Google Scholar : PubMed/NCBI
9	Deng M, Tang H, Zhou Y, Zhou M, Xiong W, Zheng Y, Ye Q, Zeng X, Liao Q, Guo X, et al: miR-216b suppresses tumor growth and invasion by targeting KRAS in nasopharyngeal carcinoma. J Cell Sci. 124:2997–3005. 2011. View Article : Google Scholar : PubMed/NCBI
10	Zehavi L, Avraham R, Barzilai A, Bar-Ilan D, Navon R, Sidi Y, Avni D and Leibowitz-Amit R: Silencing of a large microRNA cluster on human chromosome 14q32 in melanoma: Biological effects of mir-376a and mir-376c on insulin growth factor 1 receptor. Mol Cancer. 11:442012. View Article : Google Scholar : PubMed/NCBI
11	Wang L, Wu H, Wang L, Zhang H, Lu J, Liang Z and Liu T: Asporin promotes pancreatic cancer cell invasion and migration by regulating the epithelial-to-mesenchymal transition (EMT) through both autocrine and paracrine mechanisms. Cancer Lett. 398:24–36. 2017. View Article : Google Scholar : PubMed/NCBI
12	Xie X, Li YS, Xiao WF, Deng ZH, He HB, Liu Q and Luo W: MicroRNA-379 inhibits the proliferation, migration and invasion of human osteosarcoma cells by targetting EIF4G2. Biosci Rep. 37:BSR20160542. 2017. View Article : Google Scholar
13	Khan S, Brougham CL, Ryan J, Sahrudin A, O'Neill G, Wall D, Curran C, Newell J, Kerin MJ and Dwyer RM: miR-379 regulates cyclin B1 expression and is decreased in breast cancer. PLoS One. 8:e687532013. View Article : Google Scholar : PubMed/NCBI
14	Wilson SH, Bailey AM, Nourse CR, Mattei MG and Byrne JA: Identification of MAL2, a novel member of the mal proteolipid family, though interactions with TPD52-like proteins in the yeast two-hybrid system. Genomics. 76:81–88. 2001. View Article : Google Scholar : PubMed/NCBI
15	Cao Q, Chen J, Zhu L, Liu Y, Zhou Z, Sha J, Wang S and Li J: A testis-specific and testis developmentally regulated tumor protein D52 (TPD52)-like protein TPD52L3/hD55 interacts with TPD52 family proteins. Biochem Biophys Res Commun. 344:798–806. 2006. View Article : Google Scholar : PubMed/NCBI
16	Byrne JA, Frost S, Chen Y and Bright RK: Tumor protein D52 (TPD52) and cancer-oncogene understudy or understudied oncogene? Tumour Biol. 35:7369–7382. 2014. View Article : Google Scholar : PubMed/NCBI
17	Wang Z, Sun J, Zhao Y, Guo W, Lv K and Zhang Q: Lentivirus-mediated knockdown of tumor protein D52-like 2 inhibits glioma cell proliferation. Cell Mol Biol(Noisy-le-grand). 60:39–44. 2014.PubMed/NCBI
18	Pan ZY, Yang Y, Pan H, Zhang J, Liu H, Yang Y, Huang G, Yin L, Huang J and Zhou WP: Lentivirus-mediated TPD52L2 depletion inhibits the proliferation of liver cancer cells in vitro. Int J Clin Exp Med. 8:2334–2341. 2015.PubMed/NCBI
19	Inoguchi S, Seki N, Chiyomaru T, Ishihara T, Matsushita R, Mataki H, Itesako T, Tatarano S, Yoshino H, Goto Y, et al: Tumour-suppressive microRNA-24-1 inhibits cancer cell proliferation through targeting FOXM1 in bladder cancer. FEBS Lett. 588:3170–3179. 2014. View Article : Google Scholar : PubMed/NCBI
20	Han G, Fan M and Zhang X: microRNA-218 inhibits prostate cancer cell growth and promotes apoptosis by repressing TPD52 expression. Biochem Biophys Res Commun. 456:804–809. 2015. View Article : Google Scholar : PubMed/NCBI
21	Li G, Yao L, Zhang J, Li X, Dang S, Zeng K, Zhou Y and Gao F: Tumor-suppressive microRNA-34a inhibits breast cancer cell migration and invasion via targeting oncogenic TPD52. Tumour Biol. 37:7481–7491. 2016. View Article : Google Scholar : PubMed/NCBI
22	Goto Y, Nishikawa R, Kojima S, Chiyomaru T, Enokida H, Inoguchi S, Kinoshita T, Fuse M, Sakamoto S, Nakagawa M, et al: Tumour-suppressive microRNA-224 inhibits cancer cell migration and invasion via targeting oncogenic TPD52 in prostate cancer. FEBS Lett. 588:1973–1982. 2014. View Article : Google Scholar : PubMed/NCBI
23	Jin H, Yu Y, Hu Y, Lu C, Li J, Gu J, Zhang L, Huang H, Zhang D, Wu XR, et al: Divergent behaviors and underlying mechanisms of cell migration and invasion in non-metastatic T24 and its metastatic derivative T24T bladder cancer cell lines. Oncotarget. 6:522–536. 2015. View Article : Google Scholar : PubMed/NCBI
24	Ren Y, Qiu H, Yuan Y, Ye J, Tian Y, Wen B, Zhang W and Li Q: Evaluation of 7th edition of AJCC staging system for nasopharyngeal carcinoma. J Cancer. 8:1665–1672. 2017. View Article : Google Scholar : PubMed/NCBI
25	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
26	Baer C, Claus R and Plass C: Genome-wide epigenetic regulation of miRNAs in cancer. Cancer Res. 73:473–477. 2013. View Article : Google Scholar : PubMed/NCBI
27	Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev. 15:1765–1777. 2006. View Article : Google Scholar : PubMed/NCBI
28	Li Z, Shen J, Chan MT and Wu WK: MicroRNA-379 suppresses osteosarcoma progression by targeting PDK1. J Cell Mol Med. 21:315–323. 2017. View Article : Google Scholar : PubMed/NCBI
29	Gururajan M, Josson S, Chu GC, Lu CL, Lu YT, Haga CL, Zhau HE, Liu C, Lichterman J, Duan P, et al: miR-154* and miR-379 in the DLK1-DIO3 microRNA mega-cluster regulate epithelial to mesenchymal transition and bone metastasis of prostate cancer. Clin Cancer Res. 20:6559–6569. 2014. View Article : Google Scholar : PubMed/NCBI
30	Iorio MV, Ferracin M, Liu CG, Veronese A, Spizzo R, Sabbioni S, Magri E, Pedriali M, Fabbri M, Campiglio M, et al: MicroRNA gene expression deregulation in human breast cancer. Cancer Res. 65:7065–7070. 2005. View Article : Google Scholar : PubMed/NCBI
31	Bhattacharyya SN, Habermacher R, Martine U, Closs EI and Filipowicz W: Relief of microRNA-mediated translational repression in human cells subjected to stress. Cell. 125:1111–1124. 2006. View Article : Google Scholar : PubMed/NCBI
32	Shehata M, Bièche I, Boutros R, Weidenhofer J, Fanayan S, Spalding L, Zeps N, Byth K, Bright RK, Lidereau R and Byrne JA: Nonredundant functions for tumor protein D52-like proteins support specific targeting of TPD52. Clin Cancer Res. 14:5050–5060. 2008. View Article : Google Scholar : PubMed/NCBI
33	Byrne JA, Maleki S, Hardy JR, Gloss BS, Murali R, Scurry JP, Fanayan S, Emmanuel C, Hacker NF, Sutherland RL, et al: MAL2 and tumor protein D52 (TPD52) are frequently overexpressed in ovarian carcinoma, but differentially associated with histological subtype and patient outcome. BMC Cancer. 10:4972010. View Article : Google Scholar : PubMed/NCBI
34	Dasari C, Yaghnam DP, Walther R and Ummanni R: Tumor protein D52 (isoform 3) contributes to prostate cancer cell growth via targeting nuclear factor-κB transactivation in LNCaP cells. Tumour Biol. 39:10104283176983822017. View Article : Google Scholar : PubMed/NCBI