BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures

Yan,Xu; Zhou,Zhenhua; Guo,Lixin; Zeng,Zhaochi; Guo,Zhongkai; Shao,Qingdong; Xu,Weidong

doi:10.3892/etm.2018.6339

BMP7‑overexpressing bone marrow‑derived mesenchymal stem cells (BMSCs) are more effective than wild‑type BMSCs in healing fractures

Authors:
- Xu Yan
- Zhenhua Zhou
- Lixin Guo
- Zhaochi Zeng
- Zhongkai Guo
- Qingdong Shao
- Weidong Xu
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Affiliations: Department of Orthopedics, The 455th Hospital of PLA, Shanghai 200052, P.R. China, Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China, Department of Orthopedics, The 169th Hospital of PLA, Hengyang, Hunan 421002, P.R. China, Department of Orthopedics, Changhai Hospital Affiliated to The Second Military Medical University, Shanghai 200433, P.R. China
Published online on: June 21, 2018 https://doi.org/10.3892/etm.2018.6339
Pages: 1381-1388

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Abstract

Bone fractures are a worldwide public health concern. Previous studies have demonstrated that bone morphogenetic protein‑7 (BMP7) gene transfer or mesenchymal stem cells (MSCs) transplantation may be a promising novel therapeutic approach. Therefore, the aim of the present study was to observe the effect of bone BMP7 transfer to MSCs on fracture healing. Bone marrow‑derived MSCs (BMSCs) from New Zealand white rabbits were isolated and identified using flow cytometry. A recombinant BMP7 overexpressing adenovirus vector (Adv) was constructed and transfected into BMSCs. The expression of BMP7 was detected by reverse transcription‑polymerase chain reaction, immunofluorescence and western blotting. The present study additionally investigated the effect of BMP7 on the differentiation capacity of BMSCs. Finally, tissue‑engineered bone was created with support material to verify the effect of BMP7‑BMSCs on fracture healing. The results demonstrated that the expression of BMP7 was increased at the mRNA and protein levels in BMSCs following transfection with BMP7 overexpressing Adv. The results additionally demonstrated that the expression of BMP7 enhanced the differentiation capacity of bone marrow mesenchymal stem cells and had a promotional effect on fracture healing. Overall, these data suggest that Adv‑BMP7 is useful for introducing foreign genes into BMSCs and will be a powerful gene therapy tool for bone regeneration and other tissue engineering applications in the future.

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