Open Access

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

  • Authors:
    • Numan Karaarslan
    • Ahmet Guray Batmaz
    • Ibrahim Yilmaz
    • Hanefi Ozbek
    • Tezcan Caliskan
    • Duygu Yasar Sirin
    • Necati Kaplan
    • Kadir Oznam
    • Ozkan Ates
  • View Affiliations

  • Published online on: June 26, 2018     https://doi.org/10.3892/etm.2018.6351
  • Pages: 1647-1654
  • Copyright: © Karaarslan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑steroidal anti‑inflammatory drugs (NSAIDs) that are applied through oral, injectable or topical routes have been widely used in painful and inflammatory musculoskeletal diseases. The current study aimed to determine whether naproxen, an aryl acetic acid derivative with analgesic and anti‑inflammatory effects, has a toxic effect on human chondrocytes. Samples containing monolayer primary chondrocyte cultures were prepared following resection from osteochondral tissues obtained from patients with gonarthrosis. Cell viability, toxicity and proliferation and levels of stage‑specific embryonic antigen‑1, a precursor to human prechondrocytes, were evaluated spectrophotometrically. The results from the untreated control group were compared with those of the study groups, where naproxen was administered in varying doses (1‑1,000 µM). Surface morphologies of the cells were compared using inverted light and environmental scanning electron microscopy. Treatment groups were compared by analysis of variance with Tukey's honest difference post hoc test. P<0.01 was considered to indicate a statistically significant difference. The research revealed significant changes to proliferation and differentiation of chondrocytes in all treatment groups (P<0.01). Naproxen was demonstrated to suppress chondrocyte proliferation and differentiation, which may be an important factor to consider when prescribing this medication to patients.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Karaarslan N, Batmaz AG, Yilmaz I, Ozbek H, Caliskan T, Yasar Sirin D, Kaplan N, Oznam K and Ates O: Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue. Exp Ther Med 16: 1647-1654, 2018.
APA
Karaarslan, N., Batmaz, A.G., Yilmaz, I., Ozbek, H., Caliskan, T., Yasar Sirin, D. ... Ates, O. (2018). Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue. Experimental and Therapeutic Medicine, 16, 1647-1654. https://doi.org/10.3892/etm.2018.6351
MLA
Karaarslan, N., Batmaz, A. G., Yilmaz, I., Ozbek, H., Caliskan, T., Yasar Sirin, D., Kaplan, N., Oznam, K., Ates, O."Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue". Experimental and Therapeutic Medicine 16.3 (2018): 1647-1654.
Chicago
Karaarslan, N., Batmaz, A. G., Yilmaz, I., Ozbek, H., Caliskan, T., Yasar Sirin, D., Kaplan, N., Oznam, K., Ates, O."Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1647-1654. https://doi.org/10.3892/etm.2018.6351