Open Access

Effect of silencing lncRNATUG1 on rapamycin‑induced inhibition of endothelial cell proliferation and migration

  • Authors:
    • Xue Gao
    • Tao Zhang
    • Xi‑Yun Zeng
    • Guo‑Jian Li
    • Ling‑Juan Du
    • Zhen‑Huan Ma
    • Jia Wan
    • Yong Yang
  • View Affiliations

  • Published online on: June 26, 2018     https://doi.org/10.3892/etm.2018.6352
  • Pages: 1891-1899
  • Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Angiogenesis refers to the formation of new blood vessels from existing blood vessels. The proliferation and migration of endothelial cells serves a key function in this process. Previous research has demonstrated that rapamycin suppresses endothelial cell proliferation and migration, as well as angiogenesis. However, the mechanism by which rapamycin inhibits the proliferation and migration of endothelial cells remains unclear. Long noncoding RNAs (lncRNAs) serve a key function in the regulation of endothelial cell function. The aim of the current study was to investigate whether lncRNA taurine upregulated 1 (lncRNATUG1) is involved in rapamycin‑induced inhibition of proliferation and migration in human umbilical vein endothelial cells (HUVECs). Reverse transcription quantitative polymerase chain reaction results indicated that the expression of lncRNATUG1 was upregulated in HUVECs that had been cultured with rapamycin. Subsequently, HUVECs were transfected with siRNAs and CCK‑8 assays were performed to detect cell proliferation; additionally, flow cytometry was employed to detect cell apoptosis, and wound healing assays were performed to investigate cell migration. The results demonstrated that rapamycin suppressed the proliferation and migration of HUVECs, and promoted the apoptosis of HUVECs. In addition, rapamycin downregulated the expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)‑2 and MMP‑9 in HUVECs. However, silencing of lncRNATUG1 was revealed to attenuate rapamycin‑induced inhibition of cellular proliferation and migration of HUVECs, as well as upregulating the expression of VEGF, MMP2 and MMP‑9. These results suggested that lncRNATUG1 regulates rapamycin‑induced inhibition of endothelial cell proliferation and migration. Therefore, lncRNATUG1 may serve a key function in rapamycin‑induced inhibition of endothelial cell proliferation and migration.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Gao X, Zhang T, Zeng XY, Li GJ, Du LJ, Ma ZH, Wan J and Yang Y: Effect of silencing lncRNATUG1 on rapamycin‑induced inhibition of endothelial cell proliferation and migration. Exp Ther Med 16: 1891-1899, 2018.
APA
Gao, X., Zhang, T., Zeng, X., Li, G., Du, L., Ma, Z. ... Yang, Y. (2018). Effect of silencing lncRNATUG1 on rapamycin‑induced inhibition of endothelial cell proliferation and migration. Experimental and Therapeutic Medicine, 16, 1891-1899. https://doi.org/10.3892/etm.2018.6352
MLA
Gao, X., Zhang, T., Zeng, X., Li, G., Du, L., Ma, Z., Wan, J., Yang, Y."Effect of silencing lncRNATUG1 on rapamycin‑induced inhibition of endothelial cell proliferation and migration". Experimental and Therapeutic Medicine 16.3 (2018): 1891-1899.
Chicago
Gao, X., Zhang, T., Zeng, X., Li, G., Du, L., Ma, Z., Wan, J., Yang, Y."Effect of silencing lncRNATUG1 on rapamycin‑induced inhibition of endothelial cell proliferation and migration". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1891-1899. https://doi.org/10.3892/etm.2018.6352