In vitro culture and biological properties of broiler adipose‑derived stem cells

Lu,Tengfei; Pei,Wenhua; Wang,Kunfu; Zhang,Shuang; Chen,Fenghao; Wu,Yangnan; Guan,Weijun

doi:10.3892/etm.2018.6445

In vitro culture and biological properties of broiler adipose‑derived stem cells

Authors:
- Tengfei Lu
- Wenhua Pei
- Kunfu Wang
- Shuang Zhang
- Fenghao Chen
- Yangnan Wu
- Weijun Guan
View Affiliations

Affiliations: Department of Animal Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, P.R. China, College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang 150040, P.R. China, Scientific Experiment Research Center, Harbin Sport University, Harbin, Heilongjiang 150008, P.R. China, College of Human Movement Science, Harbin Sport University, Harbin, Heilongjiang 150008, P.R. China
Published online on: July 16, 2018 https://doi.org/10.3892/etm.2018.6445
Pages: 2399-2407
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

In the past 10 years, adipose‑derived stem cells (ADSCs) have been applied due to their pluripotency. Experimental tissues have been frequently obtained from mammals, including rabbits, mice and humans, but rarely from broilers, Gallus gallus domesticus. In the present study, ADSCs were obtained from 20‑day‑old broiler embryos. Primary ADSCs were sub‑cultured to passage 37 in vitro. The surface markers of ADSCs, namely CD29, CD31, CD44, CD71 and CD73, were detected by reverse transcription polymerase chain reaction and immunofluorescence assays. The result indicated that CD29, CD44, CD71 and CD73 were expressed on the surface of cells at various passages, but not CD31. The growth curve of cells at the different passages had a typical sigmoidal shape. Furthermore, ADSCs were successfully induced to differentiate into osteoblasts, adipocytes and hepatocyte‑like cells. The results denote that the ADSCs isolated from broilers have similar biological properties to those of ADSCs obtained from other animals. The present study provided a theoretical and experimental foundation for the use of poultry as a source of stem cells, and laid a foundation for adipose tissue engineering and strategies in regenerative medicine.

View Figures

View References

1	Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP and Hedrick MH: Multilineage cells from human adipose tissue: Implications for cell-based therapies. Tissue Eng. 7:211–228. 2001. View Article : Google Scholar : PubMed/NCBI
2	Rangwala SM and Lazar MA: Transcriptional control of adipogenesis. Ann Rev Nutri. 20:535–559. 2000. View Article : Google Scholar
3	Deslex S, Negrel R, Vannier C, Etienne J and Ailhaud G: Differentiation of human adipocyte precursors in a chemically defined serum-free medium. Int J Obes. 11:19–27. 1987.PubMed/NCBI
4	Hauner H, Entenmann G, Wabitsch M, Gaillard D, Ailhaud G, Negrel R and Pfeiffer EF: Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells cultured in a chemically defined medium. J Clin Invest. 84:1663–1670. 1989. View Article : Google Scholar : PubMed/NCBI
5	Halvorsen YD, Bond A, Sen A, Franklin DM, Lea-Currie YR, Sujkowski D, Ellis PN, Wilkison WO and Gimble JM: Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. Metabolism. 50:407–413. 2001. View Article : Google Scholar : PubMed/NCBI
6	Deliloglu-Gurhan SI, Vatansever HS, Ozdal-Kurt F and Tuglu I: Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system. Acta Histochem. 108:49–57. 2006. View Article : Google Scholar : PubMed/NCBI
7	Tashiro K, Kondo A, Kawabata K, Sakurai H, Sakurai F, Yamanishi K, Hayakawa T and Mizuguchi H: Efficient osteoblast differentiation from mouse bone marrow stromal cells with polylysin-modified adenovirus vectors. Biochem Bioph Res Commun. 379:127–132. 2009. View Article : Google Scholar
8	Guilak F, Lott KE, Awad HA, Cao Q, Hicok KC, Fermor B and Gimble JM: Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol. 206:229–237. 2006. View Article : Google Scholar : PubMed/NCBI
9	Gimble JM, Katz AJ and Bunnell BA: Adipose-derived stem cells for regenerative medicine. Circ Res. 100:1249–1260. 2007. View Article : Google Scholar : PubMed/NCBI
10	Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble JM and Bunnell BA: Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem. 99:1285–1297. 2006. View Article : Google Scholar : PubMed/NCBI
11	Naghdi M, Tiraihi T, Namin SA and Arabkheradmand J: Transdifferentiation of bone marrow stromal cells into cholinergic neuronal phenotype: A potential source for cell therapy in spinal cord injury. Cytotherapy. 11:137–152. 2009. View Article : Google Scholar : PubMed/NCBI
12	Zhu Y, Liu T, Song K, Fan X, Ma X and Cu Z: Adipose-derived stem cell: A better stem cell than BMSC. Cell Biochem Funct. 26:664–675. 2008. View Article : Google Scholar : PubMed/NCBI
13	Vachkova E, Bosnakovski D, Yonkova P, Grigorova N, Ivanova Zh, Todorov P, Penchev G, Milanova A, Simeonova G, Stanilova S and Georgiev IP: Adipogenic potential of stem cells derived from rabbit subcutaneous and visceral adipose tissue in vitro. In Vitro Cell Dev Biol Anim. 52:829–837. 2016. View Article : Google Scholar : PubMed/NCBI
14	Jiang Z, Harrison DE, Parsons ME, McClatchy S, Jacobs L and Pazdro R: Heritability of in vitro phenotypes exhibited by murine adipose-derived stromal cells. Mamm Genome. 27:460–468. 2016. View Article : Google Scholar : PubMed/NCBI
15	Jung HG, Ahn EK, Lee JH, Kim YH, Leem SH, Heo J and Kim H: Effects of harvesting sites and ages on adipose tissue-derived stem cells in rat. Tissue Eng Regen Med. 11:137–142. 2014. View Article : Google Scholar
16	Madonna R, Geng YJ and De Caterina R: Adipose tissue-derived stem cells characterization and potential for cardiovascular repair. Arterioscl Throm Vasc Biol. 29:1723–1729. 2009. View Article : Google Scholar
17	Rodbell M: The metabolism of isolated fat cells. IV. Regulation of release of protein by lipolytic hormones and insulin. J Biol Chem. 241:3909–3917. 1966.PubMed/NCBI
18	Rodbell M and Jones AB: Metabolism of isolated fat cells. 3. The similar inhibitory action of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on lipolysis stimulated by lipolytic hormones and theophylline. J Biol Chem. 241:140–142. 1966.PubMed/NCBI
19	Yoshimura H, Muneta T, Nimura A, Yokoyama A, Koga H and Sekiya I: Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle. Cell Tissue Res. 327:449–462. 2007. View Article : Google Scholar : PubMed/NCBI
20	Jing W, Lin Y, Wu L, Li X, Nie X, Liu L, Tang W, Zheng X and Tian W: Ectopic adipogenesis of preconditioned adipose-derived stromal cells in an alginate system. Cell Tissue Res. 330:567–572. 2007. View Article : Google Scholar : PubMed/NCBI
21	Unger RH, Scherer PE and Holland WL: Dichotomous roles of leptin and adiponectin as enforcers against lipotoxicity during feast and famine. Mol Biol Cell. 24:3011–3015. 2013. View Article : Google Scholar : PubMed/NCBI
22	Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY and Shi S: Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 364:149–155. 2004. View Article : Google Scholar : PubMed/NCBI
23	Jang HJ, Cho KS, Park HY and Roh HJ: Adipose tissue-derived stem cells for cell therapy of airway allergic diseases in mouse. Acta Histochem. 113:501–507. 2011. View Article : Google Scholar : PubMed/NCBI
24	Conget PA and Minguell JJ: Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol. 181:67–73. 1999. View Article : Google Scholar : PubMed/NCBI
25	Haynesworth SE, Baber MA and Caplan AI: Cytokine expression by human marrow-derived mesenchymal progenitor cells in vitro: Effects of dexamethasone and IL-1 alpha. J Cell Physiol. 166:585–592. 1996. View Article : Google Scholar : PubMed/NCBI
26	Levy JE, Jin O, Fujiwara Y, Kuo F and Andrews NC: Transferrin receptor is necessary for development of erythrocytes and the nervous system. Nat Genet. 21:396–399. 1999. View Article : Google Scholar : PubMed/NCBI
27	Gong X, Hou L, Bai C, Jin D, He X, Guan W and Ma Y: Isolation and biological characteristics of chicken adipose-derived progenitor Cells. DNA Cell Biol. 30:453–460. 2011. View Article : Google Scholar : PubMed/NCBI
28	Nogami M, Tsuno H, Koike C, Okabe M, Yoshida T, Seki S, Matsui Y, Kimura T and Nikaido T: Isolation and characterization of human amniotic mesenchymal stem cells and their chondrogenic differentiation. Transplantation. 93:1221–1228. 2012. View Article : Google Scholar : PubMed/NCBI
29	Nakagami H, Morishita R, Maeda K, Kikuchi Y, Ogihara T and Kaneda Y: Adipose tissue-derived stromal cells as a novel option for regenerative cell therapy. J Atheroscler Thromb. 13:77–81. 2006. View Article : Google Scholar : PubMed/NCBI
30	Uzbas F, May ID, Parisi AM, Thompson SK, Kaya A, Perkins AD and Memili E: Molecular physiognomies and applications of adipose-derived stem cells. Stem Cell Rev Rep. 11:298–308. 2015. View Article : Google Scholar