Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene

Huang,Xiaoxi; Lyu,Jin

doi:10.3892/etm.2018.6504

Tumor suppressor function of miR‑483‑3p on breast cancer via targeting of the cyclin E1 gene

Authors:
- Xiaoxi Huang
- Jin Lyu
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Affiliations: Department of Breast, Fujian Provincial Maternity and Children Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Department of Surgical Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
Published online on: July 23, 2018 https://doi.org/10.3892/etm.2018.6504
Pages: 2615-2620

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Abstract

microRNA (miR)‑483‑3p has previously been demonstrated to be a tumor suppressor in many types of cancer cells, however it is unknown whether miR‑483‑3p is involved in the regulation of breast cancer. Therefore, the aim of the present study was to investigate the effects of miR‑483‑3p on breast cancer. The results demonstrated that the expression of miR‑483‑3p was decreased, especially in MCF‑7 cells. The results of CCK‑8 assay and cell cycle analysis demonstrated that miR‑483‑3p significantly reduced cell proliferation and inhibited MCF‑7 cells in the G1 phase from entering the S phase (P<0.01). It was also demonstrated that Cyclin E1 (CCNE1) is a target gene of miR‑483‑3p using bioinformatics and a dual luciferase reporter assay. miR‑483‑3p inhibited the expression of CCNE1, cyclin‑dependent kinase 2, nuclear protein ataxia‑telangiectasia (NPAT) and phosphorylated NPAT. Therefore, the results indicated that miR‑483‑3p functions as a tumor suppression in breast cancer and CCNE1 is its target gene. Downstream genes of CCNE1 were also repressed by miR‑483‑3p. Therefore, these findings suggest that miR‑483‑3p is a key factor in breast cancer.

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