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MicroRNA‑744 promotes cell apoptosis via targeting B cell lymphoma‑2 in gastric cancer cell line SGC‑7901

  • Authors:
    • Jixiang Liu
    • Yanlei Wei
    • Shouyong Li
    • Yujuan Li
    • Hongxiu Liu
    • Jingmei Liu
    • Xinxing Zhu

  • View Affiliations

  • Published online on: August 13, 2018     https://doi.org/10.3892/etm.2018.6602
  • Pages: 3611-3616
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Abstract

Gastric cancer (GC) affects the health of 1,000,000 people per year worldwide; however, the biological basis of GC remains largely unknown. The current study aimed to investigate the aberrant expression of miR‑744 in GC for the effective treatment of patients with GC. Tumor and adjacent tissues were obtained from 30 patients who underwent tumor resection surgery at Dongying People's Hospital. The results of reverse transcription‑quantitative polymerase chain reaction indicated that the expression of miR‑744 was significantly decreased in tumor tissues compared with the levels in adjacent tissues. Human gastric cancer cell line SGC‑7901 was then randomly divided into three different groups, including the control, miR‑negative control (NC) and miR‑744 mimic groups. A Cell Counting Kit‑8 assay demonstrated that there was a significant decrease in the proliferation rate of SGC‑7901 cells in the miR‑744 mimics group compared with that observed in the control and miR‑NC mimics groups. In addition, flow cytometry demonstrated that apoptosis was significantly increased in the miR‑744 mimics group compared with that observed in the control and miR‑NC mimics groups. Western blotting indicated that the expression of B cell lymphoma 2 (Bcl‑2), B cell lymphoma‑2‑associated X protein and caspase‑3 protein was significantly increased, while the expression of Bcl‑2 was significantly decreased in the miR‑744 mimics group compared with the levels observed in the control and miR‑NC mimics groups. A dual‑luciferase assay verified that miR‑744 directly targeted the 3'‑untranslated region of Bcl‑2. Taken together, the present study suggested that miR‑744 serves a tumor suppressive role in GC by targeting Bcl‑2.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu J, Wei Y, Li S, Li Y, Liu H, Liu J and Zhu X: MicroRNA‑744 promotes cell apoptosis via targeting B cell lymphoma‑2 in gastric cancer cell line SGC‑7901. Exp Ther Med 16: 3611-3616, 2018.
APA
Liu, J., Wei, Y., Li, S., Li, Y., Liu, H., Liu, J., & Zhu, X. (2018). MicroRNA‑744 promotes cell apoptosis via targeting B cell lymphoma‑2 in gastric cancer cell line SGC‑7901. Experimental and Therapeutic Medicine, 16, 3611-3616. https://doi.org/10.3892/etm.2018.6602
MLA
Liu, J., Wei, Y., Li, S., Li, Y., Liu, H., Liu, J., Zhu, X."MicroRNA‑744 promotes cell apoptosis via targeting B cell lymphoma‑2 in gastric cancer cell line SGC‑7901". Experimental and Therapeutic Medicine 16.4 (2018): 3611-3616.
Chicago
Liu, J., Wei, Y., Li, S., Li, Y., Liu, H., Liu, J., Zhu, X."MicroRNA‑744 promotes cell apoptosis via targeting B cell lymphoma‑2 in gastric cancer cell line SGC‑7901". Experimental and Therapeutic Medicine 16, no. 4 (2018): 3611-3616. https://doi.org/10.3892/etm.2018.6602