Subarachnoid hemorrhage enhances the expression of TDP‑43 in the brain of experimental rats and human subjects

He,Tibiao; Zuo,Yuchun; Ai‑Zakwani,Kauthar; Luo,Jing; Zhu,Haixia; Yan,Xiao‑Xin; Liu,Fei

doi:10.3892/etm.2018.6636

Subarachnoid hemorrhage enhances the expression of TDP‑43 in the brain of experimental rats and human subjects

Authors:
- Tibiao He
- Yuchun Zuo
- Kauthar Ai‑Zakwani
- Jing Luo
- Haixia Zhu
- Xiao‑Xin Yan
- Fei Liu
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Affiliations: Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Neurology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China, Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China
Published online on: August 21, 2018 https://doi.org/10.3892/etm.2018.6636
Pages: 3363-3368
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The transactive response DNA‑binding protein of 43 (TDP‑43) may be involved in neurodegenerative disease and in the response to brain injury; however, alterations in the expression of TDP‑43 following subarachnoid hemorrhage (SAH) require further investigation. The present study reported a notable elevation in the expression of TDP‑43 within the cerebrospinal fluid (CSF) of patients with aneurysmal SAH and increased brain expression of TDP‑43 in a rat model of SAH. The TDP‑43 protein and a derivative migrated at 43 and 24 kDa, respectively, as observed via the immunoblotting of concentrated CSF samples obtained from patients with SAH; no signal was detected in the CSF from healthy controls. SAH in rats was induced by intravascular suture puncture. The expression levels of TDP‑43 in rat cortical lysates following SAH were increased at 0.5 h, peaked at 48 h and remained significantly elevated at 72 h post‑injury, compared with sham controls. TDP‑43 immunolabeling indication localization within neurons, astrocytes and microglia in the experimental rats. Collectively, the findings of the present study indicated the early involvement of TDP‑43 in the brain in response to SAH, and that expression levels of TDP‑43 in the CSF may serve as a prognostic biomarker among patients with this condition.

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