miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Tian,Jing; Shen,Rui; Yan,Yuzhang; Deng,Liehua

doi:10.3892/etm.2018.6679

miR‑186 promotes tumor growth in cutaneous squamous cell carcinoma by inhibiting apoptotic protease activating factor‑1

Authors:
- Jing Tian
- Rui Shen
- Yuzhang Yan
- Liehua Deng
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Affiliations: Department of Dermatology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Plastic Cosmetic Surgery, The First People's Hospital of Foshan, Foshan, Guangdong 528000, P.R. China, Department of Psychiatry, Tianhe District Chronic Disease Prophylactic‑Therapeutic Institution, Guangzhou, Guangdong 510599, P.R. China
Published online on: September 3, 2018 https://doi.org/10.3892/etm.2018.6679
Pages: 4010-4018
Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cutaneous squamous cell carcinoma (cSCC) accounts for 20% of non‑melanoma skin cancer worldwide. MicroRNAs (miRNAs or miRs) are a subtype of non‑coding RNA associated with the progression of various types of human cancer. MiR‑186 has been demonstrated to act as an oncogene in human tumors. However, the role of miR‑186 in cSCC remains unclear. The expression of miR‑186 and apoptotic protease activating factor 1 (APAF1) was examined using reverse transcription‑quantitative polymerase chain reaction, western blotting and immunofluorescence. The correlation between miR‑186 and APAF1 was determined using a dual‑luciferase assay. Mimics or inhibitors of miR‑186 were transfected into A‑431 cells to establish cell lines with overexpressed or knocked‑down miR‑186, respectively. EdU staining and colony formation assays were performed to detect cell proliferation. Transwell and wound‑healing assays were performed to analyze cell invasion and migration, respectively. Hoechst staining and flow cytometry were performed to assess cell apoptosis and cell cycle distribution. MiR‑186 expression was significantly increased, while APAF1 expression was significantly decreased in cSCC tissues compared with the controls. An miR‑186 binding site was predicted in APAF1 and their expression was negatively correlated in cSCC tissues. Cell proliferation, invasion and migration were significantly enhanced in the miR‑186‑overexpressed A‑431 cells and attenuated in miR‑186 knockdown cells compared with the control. APAF1 expression was regulated by miR‑186, while APAF1 knockdown significantly promoted cell invasion and inhibited cell apoptosis. In summary, the results of the present study indicate that miR‑186 serves as an oncogene in cSCC by inhibiting APAF1.

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