Abnormal expression of miR‑4784 in chondrocytes of osteoarthritis and associations with chondrocyte hyperplasia

Liu,Jing; Yu,Qiaolong; Ye,Yanhui; Yan,Yan; Chen,Xin

doi:10.3892/etm.2018.6739

Abnormal expression of miR‑4784 in chondrocytes of osteoarthritis and associations with chondrocyte hyperplasia

Authors:
- Jing Liu
- Qiaolong Yu
- Yanhui Ye
- Yan Yan
- Xin Chen
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Affiliations: Department of Nosocomial Infection Control, The 174th Hospital of Chinese PLA, Chenggong Hospital Affiliated to Medical College of Xiamen University, Xiamen, Fujian 361003, P.R. China, Department of General Dentistry, The 174th Hospital of Chinese PLA, Chenggong Hospital Affiliated to Medical College of Xiamen University, Xiamen, Fujian 361003, P.R. China
Published online on: September 14, 2018 https://doi.org/10.3892/etm.2018.6739
Pages: 4690-4694

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Abstract

The aim of the present study was to assess the expression of microRNA (miRNA)‑4784 in the chondrocytes of early osteoarthritis (OA) and to determine the effect of double‑stranded (ds)‑miRNA‑4784 transfection on chondrocyte function. Following the construction of an OA rabbit model, normal chondrocytes (normal control group), OA chondrocytes obtained 4 weeks after modeling (OA at week 4 group) and 8 weeks after modeling (OA at week 8 group) were used. The relative expression of miRNA‑4784 in each group was detected using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Western blotting was performed to measure the expression of type II collagen (Col2a1) and matrix metalloproteinase (MMP)‑3 in each group with or without ds‑miRNA‑4784 transfection. The results revealed that the levels of miR‑4784 in groups OA at week 4 and 8 were significantly lower than that of normal control group (P<0.05). It was also demonstrated that Col2a1 mRNA expression levels in groups OA at week 4 and 8 were 49 and 38% of that in the normal control group, respectively. Furthermore, MMP‑3 mRNA expression levels increased by 3.12‑ and 3.95‑fold in groups OA at week 4 and 8, respectively, compared with those in the normal control group (P<0.01). Following transfection with ds‑miRNA‑4784, Col2a1 mRNA expression levels increased by 63 and 126% compared with the levels prior to treatment in groups OA at week 4 and 8, respectively (P<0.01). The expression levels of MMP‑3 mRNA in groups OA at week 4 and 8 decreased following transfection compared with the levels prior to treatment. Col2a1 and MMP‑3 protein expression exhibited similar patterns to the mRNA expression. In summary, the results of the present study suggest that miRNA‑4784 expression is significantly reduced in early stage OA chondrocytes. Transfection with ds‑miRNA‑4784 promotes the expression of Col2a1 and inhibits the MMP‑3 expression in chondrocytes.

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