Open Access

Resveratrol protects against oxidative stress by activating the Keap‑1/Nrf2 antioxidant defense system in obese‑asthmatic rats

  • Authors:
    • Xiao‑Nan Li
    • Lu‑Yi Ma
    • Hong Ji
    • Yuan‑Hua Qin
    • Shan‑Shan Jin
    • Li‑Xin Xu
  • View Affiliations

  • Published online on: September 17, 2018     https://doi.org/10.3892/etm.2018.6747
  • Pages: 4339-4348
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the potential mechanism underlying the anti‑obesity‑asthmatic effects of resveratrol (RSV) in a rat model of obese‑asthma. Rat models of obesity and asthma were established using a high‑fat diet and the administration of ovalbumin, respectively. Rats were divided into 7 different groups: A normal control, a normal obese, a normal asthma, a normal obese + asthma, a RSV obese, a RSV asthma and a RSV obese + asthma group. Body weight, Lee index, body fat and lung histopathological changes were evaluated. Serum lipid levels were evaluated using calorimetric methods. Levels of reactive oxygen species (ROS) were examined using enzyme‑linked immunosorbent assays. Cellular antioxidant enzyme activities were measured using commercial kits. Levels of kelch‑like ECH associated protein 1 (Keap‑1) and nuclear factor erythroid 2‑related factor 2 (Nrf2) was examined using western blot analysis. The results indicated that obese and asthma rat models were successfully established. It was also demonstrated that RSV decreased fasting blood glucose in obese, asthmatic and obese‑asthmatic rats. RSV altered serum lipid levels; it significantly increased high density lipoprotein cholesterol levels and significantly decreased serum triglyceride, serum total cholesterol and very low density lipoprotein levels, compared with untreated obese, asthmatic and obese‑asthmatic rats (P<0.05). ROS levels were significantly decreased in the RSV treatment group compared with obese, asthmatic and obese‑asthmatic rats (P<0.05). RSV treatment significantly increased catalase, glutathione, glutathione peroxidase and total superoxide dismutase levels compared with untreated obese, asthmatic and obese‑asthmatic rats (P<0.05). Furthermore, RSV treatment significantly downregulated Keap‑1 and upregulated Nrf2 levels in the heart, lung and kidney tissues of rats compared with untreated controls. Therefore, the results demonstrate that RSV protects against oxidative stress by activating the Keap‑1/Nrf2 antioxidant defense system in obese‑asthmatic rat models.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li XN, Ma LY, Ji H, Qin YH, Jin SS and Xu LX: Resveratrol protects against oxidative stress by activating the Keap‑1/Nrf2 antioxidant defense system in obese‑asthmatic rats. Exp Ther Med 16: 4339-4348, 2018.
APA
Li, X., Ma, L., Ji, H., Qin, Y., Jin, S., & Xu, L. (2018). Resveratrol protects against oxidative stress by activating the Keap‑1/Nrf2 antioxidant defense system in obese‑asthmatic rats. Experimental and Therapeutic Medicine, 16, 4339-4348. https://doi.org/10.3892/etm.2018.6747
MLA
Li, X., Ma, L., Ji, H., Qin, Y., Jin, S., Xu, L."Resveratrol protects against oxidative stress by activating the Keap‑1/Nrf2 antioxidant defense system in obese‑asthmatic rats". Experimental and Therapeutic Medicine 16.6 (2018): 4339-4348.
Chicago
Li, X., Ma, L., Ji, H., Qin, Y., Jin, S., Xu, L."Resveratrol protects against oxidative stress by activating the Keap‑1/Nrf2 antioxidant defense system in obese‑asthmatic rats". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4339-4348. https://doi.org/10.3892/etm.2018.6747