Open Access

miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells

  • Authors:
    • Liang Yan
    • Jinzhu Ma
    • Yi Wang
    • Jiawei Zan
    • Zhen Wang
    • Yu Zhu
    • Yiping Zhu
    • Liefeng Ling
    • Long Cao
    • Xin Liu
    • Shu Li
    • Lei Xu
    • Zhilin Qi
    • Liuwang Nie
    • Yao Zhang
  • View Affiliations

  • Published online on: September 18, 2018     https://doi.org/10.3892/etm.2018.6752
  • Pages: 4655-4663
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The mortality rate of non‑small cell lung cancer (NSCLC) remains high worldwide. miR‑21‑5p plays an important part in many cancer types, including NSCLC. However, the effect of miR‑21‑5p in NSCLC tumorigenesis remains poorly understood. The present study investigated whether miR‑21‑5p promoted NSCLC cell proliferation in vitro. In order to study the molecular mechanism by which miR‑21‑5p contributes to NSCLC progression, three bioinformatics algorithms were used to predict the genes which miR‑21‑5p targeted. TGFBI was identfieid as a putative direct target in NSCLC cells via the luciferase reporter assay. Furthermore, miR‑21‑5p downregulated TGFBI protein expression by a post‑transcriptional mechanism via western blotting and a reverse transcription‑quantitative polymerase chain reaction analysis. Finally, TGFBI exhibited opposing effects to those of miR‑21‑5p on NSCLC cells, suggesting that miR‑21‑5p may promote cell proliferation by negative regulation of TGFBI. These results suggest miR‑21‑5p promote the proliferation of NSCLC cells via inhibiting TGFBI expression.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yan L, Ma J, Wang Y, Zan J, Wang Z, Zhu Y, Zhu Y, Ling L, Cao L, Liu X, Liu X, et al: miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells. Exp Ther Med 16: 4655-4663, 2018.
APA
Yan, L., Ma, J., Wang, Y., Zan, J., Wang, Z., Zhu, Y. ... Zhang, Y. (2018). miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells. Experimental and Therapeutic Medicine, 16, 4655-4663. https://doi.org/10.3892/etm.2018.6752
MLA
Yan, L., Ma, J., Wang, Y., Zan, J., Wang, Z., Zhu, Y., Zhu, Y., Ling, L., Cao, L., Liu, X., Li, S., Xu, L., Qi, Z., Nie, L., Zhang, Y."miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells". Experimental and Therapeutic Medicine 16.6 (2018): 4655-4663.
Chicago
Yan, L., Ma, J., Wang, Y., Zan, J., Wang, Z., Zhu, Y., Zhu, Y., Ling, L., Cao, L., Liu, X., Li, S., Xu, L., Qi, Z., Nie, L., Zhang, Y."miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4655-4663. https://doi.org/10.3892/etm.2018.6752