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Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS

  • Authors:
    • Yanyun Cao
    • Shunming Xu
    • Wei Kong
    • Haibin Cai
    • Yang Xu

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  • Published online on: October 1, 2018     https://doi.org/10.3892/etm.2018.6818
  • Pages: 4527-4536

  • Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic spontaneous urticaria (CSU) is one of the most common types of chronic urticaria (CU), with symptoms that recur easily, migrate and are refractory. It is unclear whether association between the differentiation of protein expression levels in the serum of CSU patients and the different duration of wheals exists. In the present study the samples were divided according to the duration of the wheals into group A (wheal duration <2 h) and group B (wheal duration 12‑24 h). Differentially expressed proteins in sera of CSU patients with different durations of wheals were identified and validated with isobaric tags for relative and absolute quantitation (iTRAQ) in combination with two‑dimensional liquid chromatography/tandem mass spectrometry (2D‑LC‑MS/MS). Three hundred and seventy CSU serum‑related proteins were initially identified. Among these proteins, ~30 had significant differences between the groups. According to the classification of biological functions and upregulated/downregulated values, serum amyloid A (SAA), CFL1, TPM4 and monocyte differentiation antigen (CD14) were chosen and validated by enzyme‑linked immunosorbent assay (ELISA). The expression levels of CD14 in sera were not significantly different among the groups. SAA, CFL1 and TPM4 were associated with the wheal duration in CSU patients and therefore could be considered as new potential inflammatory biomarkers associated with CSU.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cao Y, Xu S, Kong W, Cai H and Xu Y: Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS. Exp Ther Med 16: 4527-4536, 2018.
APA
Cao, Y., Xu, S., Kong, W., Cai, H., & Xu, Y. (2018). Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS. Experimental and Therapeutic Medicine, 16, 4527-4536. https://doi.org/10.3892/etm.2018.6818
MLA
Cao, Y., Xu, S., Kong, W., Cai, H., Xu, Y."Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS". Experimental and Therapeutic Medicine 16.6 (2018): 4527-4536.
Chicago
Cao, Y., Xu, S., Kong, W., Cai, H., Xu, Y."Identification and validation of differentially expressed proteins in serum of CSU patients with different duration of wheals using an iTRAQ labeling, 2D‑LC‑MS/MS". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4527-4536. https://doi.org/10.3892/etm.2018.6818