miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p expression in alveolar ridge atrophy and their roles in the proliferation and apoptosis of OMMSCs

Yang,Sefei; Guo,Jun; Zhou,Lei; Xing,Helin; Wang,Xianli; Dong,Chaofang

doi:10.3892/etm.2018.6850

miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p expression in alveolar ridge atrophy and their roles in the proliferation and apoptosis of OMMSCs

Authors:
- Sefei Yang
- Jun Guo
- Lei Zhou
- Helin Xing
- Xianli Wang
- Chaofang Dong
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Affiliations: Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Prosthodontics, School of Stomatology, Capital Medical University, Beijing 100050, P.R. China, Department of Prosthodontics, Anyang Sixth People's Hospital, Anyang, Henan 455000, P.R. China, Lab for Corrosion Control and Research Development, School of Mathematics and Physics, University of Science and Technology Beijing, Beijing 100853, P.R. China
Published online on: October 11, 2018 https://doi.org/10.3892/etm.2018.6850
Pages: 5334-5342

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Abstract

MicroRNAs (miRNAs/miRs) have key roles in various physiological and pathological processes by regulating the expression of specific genes. The identification of miRNAs involved in bone metabolism may provide insight into the expression of genes associated with the development of alveolar ridge atrophy. In the present study, the miRNA expression profiles in alveolar ridge atrophy and normal tissue samples were investigated by miRNA microarray analysis. Among the 52 differentially expressed miRNAs identified, the expression levels of 20 selected miRNAs in the alveolar ridge atrophy and normal tissue samples were verified by reverse transcription‑quantitative polymerase chain reaction. The results indicated that the expression levels of 11 miRNAs were significantly different between alveolar ridge atrophy and normal tissue samples; however, only three of them (miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p) were previously reported to be involved in bone metabolism. In vitro, miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p mimics promoted the proliferation and inhibited apoptosis of bone marrow mesenchymal stem cells from orofacial bone (OMMSCs), while antisense inhibitors of these miRNAs had the opposite effect. In conclusion, the present study indicated that these miRNAs are involved in the pathogenesis of alveolar ridge atrophy. miR‑148b‑3p, miR‑337‑5p and miR‑423‑5p promote the proliferation of OMMSCs and inhibit their apoptosis. The present results provide a novel perspective for understanding the pathogenesis of alveolar ridge atrophy.

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