Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis

Liu,Baolong; Zhang,Maoquan; Zhao,Jingming; Zheng,Mei; Yang,Hao

doi:10.3892/etm.2018.6852

Imbalance of M1/M2 macrophages is linked to severity level of knee osteoarthritis

Authors:
- Baolong Liu
- Maoquan Zhang
- Jingming Zhao
- Mei Zheng
- Hao Yang
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Affiliations: Department of Emergency Surgery, The Affiliated Qingdao Hiser Hospital of Qingdao University (Qingdao Hospital of Traditional Chinese Medicine), Qingdao, Shandong 266001, P.R. China, Department of Rheumatology, The Affiliated Qingdao Hiser Hospital of Qingdao University (Qingdao Hospital of Traditional Chinese Medicine), Qingdao, Shandong 266001, P.R. China, Department of Spine Surgery, The Affiliated Qingdao Hiser Hospital of Qingdao University (Qingdao Hospital of Traditional Chinese Medicine), Qingdao, Shandong 266001, P.R. China, Department of Internal Medicine, People's Hospital of Zhangqiu, Jinan, Shandong 250200, P.R. China
Published online on: October 11, 2018 https://doi.org/10.3892/etm.2018.6852
Pages: 5009-5014
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Macrophages, whether M1 or M2 subtype, have been found to be implicated in the pathogenesis of osteoarthritis (OA). However, no study regarding the status of M1 and M2 macrophages has been reported in knee OA. To investigate the status of M1 and M2 macrophages in knee OA, synovial fluid as well as peripheral blood were collected from 80 patients with knee OA and 80 healthy controls. Reverse transcription-quantitative PCR was used to quantitatively detect the expression of CD11c as a marker for M1 macrophages and CD206 as a marker for M2 macrophages from synovial fluids. As confirmation, flow cytometry was employed to count the number of monocytes from whole blood using the CD86 (M1) and CD163 (M2) markers on monocytes. The ratio of M1 to M2 macrophages was shown to be markedly higher in knee OA than that of control and that the ratio was significantly positively correlated with level of Kellgren-Lawrence grade in knee OA, that is, the higher the ratio the more severe the knee OA seems to be. Thus, our study presented direct evidence for the involvement of macrophages in the pathogenesis of knee OA.

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