Open Access

Endothelial PAS domain protein 1 gene hypomethylation is associated with colorectal cancer in Han Chinese

  • Authors:
    • Ranran Pan
    • Cong Zhou
    • Jie Dai
    • Xiuru Ying
    • Hang Yu
    • Jie Zhong
    • Yihan Zhang
    • Boyi Wu
    • Yiyi Mao
    • Dongping Wu
    • Jieer Ying
    • Wei Zhang
    • Shiwei Duan
  • View Affiliations

  • Published online on: October 12, 2018     https://doi.org/10.3892/etm.2018.6856
  • Pages: 4983-4990
  • Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Endothelial PAS domain‑containing protein 1 (EPAS1) serves a role in angiogenesis, which is important for the development of tumors, including colorectal cancer (CRC). The current study aimed to estimate whether EPAS1 methylation was associated with CRC. A two‑stage association study of EPAS1 methylation and CRC was conducted. In the first phase, EPAS1 methylation was evaluated in the tumor and adjacent non‑tumor tissue samples from 41 patients with sporadic CRC in Jiangsu province, China. The diagnostic value of methylation of EPAS1 for CRC in the second phase was evaluated in 79 patients with sporadic CRC and 22 normal individuals in Zhejiang province, China. The methylation assay was performed using a quantitative methylation‑specific polymerase chain reaction (qMSP) method. The percentage of methylated reference (PMR) was used to quantify the methylation level. The first‑stage results indicated that EPAS1 promoter methylation was significantly lower in CRC tumor tissues compared with 5‑cm‑para‑tumor tissues (median PMR, 0.59 vs. 1.22%; P=0.027) and 10‑cm‑para‑tumor tissues (median PMR, 0.59 vs. 1.89%; P=0.001). In addition, the second‑stage results indicated that EPAS1 promoter methylation was significantly lower in tumor tissues compared with 5‑cm‑para‑tumor tissues (median PMR, 1.91 vs. 6.25%; P=3x10‑7) and normal intestinal tissues from healthy controls (median PMR, 1.91 vs. 28.4%; P=5x10‑7). Receiver Operating Characteristic curve analysis of the second‑stage data indicated that the highest area under the curve of EPAS1 hypomethylation was 0.851 between Zhejiang CRC tissues and Zhejiang normal intestinal tissues (sensitivity, 95.5%; specificity, 60.8%).
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Pan R, Zhou C, Dai J, Ying X, Yu H, Zhong J, Zhang Y, Wu B, Mao Y, Wu D, Wu D, et al: Endothelial PAS domain protein 1 gene hypomethylation is associated with colorectal cancer in Han Chinese. Exp Ther Med 16: 4983-4990, 2018.
APA
Pan, R., Zhou, C., Dai, J., Ying, X., Yu, H., Zhong, J. ... Duan, S. (2018). Endothelial PAS domain protein 1 gene hypomethylation is associated with colorectal cancer in Han Chinese. Experimental and Therapeutic Medicine, 16, 4983-4990. https://doi.org/10.3892/etm.2018.6856
MLA
Pan, R., Zhou, C., Dai, J., Ying, X., Yu, H., Zhong, J., Zhang, Y., Wu, B., Mao, Y., Wu, D., Ying, J., Zhang, W., Duan, S."Endothelial PAS domain protein 1 gene hypomethylation is associated with colorectal cancer in Han Chinese". Experimental and Therapeutic Medicine 16.6 (2018): 4983-4990.
Chicago
Pan, R., Zhou, C., Dai, J., Ying, X., Yu, H., Zhong, J., Zhang, Y., Wu, B., Mao, Y., Wu, D., Ying, J., Zhang, W., Duan, S."Endothelial PAS domain protein 1 gene hypomethylation is associated with colorectal cancer in Han Chinese". Experimental and Therapeutic Medicine 16, no. 6 (2018): 4983-4990. https://doi.org/10.3892/etm.2018.6856