Oligo‑fucoidan improved unbalance the Th1/Th2 and Treg/Th17 ratios in asthmatic patients: An ex vivo study

Yang,Chao‑Huei; Tian,Jing‑Jing; Ko,Wang‑Sheng; Shih,Chia‑Ju; Chiou,Ya‑Ling

doi:10.3892/etm.2018.6939

Oligo‑fucoidan improved unbalance the Th1/Th2 and Treg/Th17 ratios in asthmatic patients: An ex vivo study

Authors:
- Chao‑Huei Yang
- Jing‑Jing Tian
- Wang‑Sheng Ko
- Chia‑Ju Shih
- Ya‑Ling Chiou
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Affiliations: Department of Internal Medicine, Kuang‑Tien General Hospital, Taichung 43302, Taiwan R.O.C., Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, Taichung 43302, Taiwan R.O.C.
Published online on: November 7, 2018 https://doi.org/10.3892/etm.2018.6939
Pages: 3-10
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

An imbalance in the helper T cells (Th)1/Th2 and regulatory T cells (Tregs)/Th17 ratios is believed to play a key role in asthmatic inflammatory responses. Fucoidan reportedly reduces the production of inflammatory factors. Nutritional intervention is an important tool in decreasing the severity of asthmatic disease. This study aimed to investigate the beneficial roles of oligo‑fucoidan in balancing the T cell subtype ratios and reducing airway inflammation ex vivo. Peripheral blood mononuclear cells (PBMCs) were collected from 30 asthmatic subjects and 15 healthy subjects. Harvested PBMCs were stimulated and treated with or without oligo‑fucoidan (100 or 500 µg/ml) for 48 h. Cell surface and intracellular cytokine markers were examined by flow cytometry. The pro‑inflammatory factors in plasma and culture supernatants were measured using ELISA kits. We found that oligo‑fucoidan increases the proportion of Th1 and Treg cells, but did not affect the proportion of Th2 and Th17 cells. Oligo‑fucoidan also increased the levels of interferon‑γ and interleukin‑10. Thus, we concluded that oligo‑fucoidan might improve the imbalance in Th1/Th2 and Treg/Th17 ratios to reduce airway inflammation, which could be a potential adjuvant therapy for allergic asthma.

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