ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation

  • Authors:
    • Wu Ye
    • Zhijun Li
    • Tingyu Tang
    • Jianzong Du
    • Xiaoxi Zhou
    • Haiyan Wu
    • Xuefang Li
    • Guangyue Qin
  • View Affiliations

  • Published online on: November 30, 2018     https://doi.org/10.3892/etm.2018.7040
  • Pages: 817-823
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the current study was to assess the underlying mechanism of endoplasmic reticulum protein 29 (ERp29) in lung adenocarcinoma chemosensitivity to gemcitabine. Western blot analysis was performed to detect ERp29 expression following lung adenocarcinoma cell treatment with gemcitabine. The effects of gemcitabine in combination with ERp29 siRNA on cell apoptosis, cell cycle and heat shock protein 27 (HSP27) expression were assessed. The results demonstrated that ERp29 expression was increased on exposure to gemcitabine. The apoptotic rate of lung adenocarcinoma cells were also increased following gemcitabine treatment and the combined application of gemcitabine and ERp29 siRNA synergistically increased apoptotic rates further. It was also revealed that gemcitabine and ERp29 siRNA synergistically increased the ratio of phosphorylated to total HSP27 protein. In addition, downregulation of HSP27 significantly reduced lung adenocarcinoma chemosensitivity to gemcitabine. These data indicate that ERp29 affects lung adenocarcinoma cell chemosensitivity to gemcitabine by regulating phosphorylated HSP27. ERp29 is a novel target, which may be used to enhance the therapeutic effect of lung adenocarcinoma treatment with gemcitabine.
View Figures
View References

Related Articles

Journal Cover

January-2019
Volume 17 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ye W, Li Z, Tang T, Du J, Zhou X, Wu H, Li X and Qin G: ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation. Exp Ther Med 17: 817-823, 2019
APA
Ye, W., Li, Z., Tang, T., Du, J., Zhou, X., Wu, H. ... Qin, G. (2019). ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation. Experimental and Therapeutic Medicine, 17, 817-823. https://doi.org/10.3892/etm.2018.7040
MLA
Ye, W., Li, Z., Tang, T., Du, J., Zhou, X., Wu, H., Li, X., Qin, G."ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation". Experimental and Therapeutic Medicine 17.1 (2019): 817-823.
Chicago
Ye, W., Li, Z., Tang, T., Du, J., Zhou, X., Wu, H., Li, X., Qin, G."ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation". Experimental and Therapeutic Medicine 17, no. 1 (2019): 817-823. https://doi.org/10.3892/etm.2018.7040