MicroRNA‑375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1

Xu,Hui; Jiang,Jialong; Zhang,Jingjun; Cheng,Liang; Pan,Song; Li,Yuanhai

doi:10.3892/etm.2018.7106

MicroRNA‑375 inhibits esophageal squamous cell carcinoma proliferation through direct targeting of SP1

Authors:
- Hui Xu
- Jialong Jiang
- Jingjun Zhang
- Liang Cheng
- Song Pan
- Yuanhai Li
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Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Anesthesiology, The Third People's Hospital of Bengbu, Bengbu, Anhui 233000, P.R. China
Published online on: December 17, 2018 https://doi.org/10.3892/etm.2018.7106
Pages: 1509-1516
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Several studies have shown that microRNA‑375 (miR‑375) is frequently downregulated in several types of human cancer including gastric cancer, colorectal cancer and oral squamous cell carcinoma. However, the role of miR‑375 in human esophageal cancer remains unknown. In the current study, the expression level of miR‑375 was analyzed in 43 esophageal squamous cell carcinoma (ESCC) tissue and matched adjacent normal tissue samples from patients with ESCC by reverse transcription‑quantitative polymerase chain reaction. In addition, the expression level of miR‑375 was analyzed in ESCC cell lines (KYSE450 and KYSE150) and the human esophageal epithelial cell line Het‑1A by the same method. The expression level of miR‑375 was significantly downregulated in ESCC tissue samples and cell lines compared with adjacent normal tissue samples and the human esophageal epithelial cell line, respectively. The effect of miR‑375 on ESCC cell proliferation was detected by cell counting kit‑8 (CCK‑8) and colony formation assays. miR‑375 overexpression significantly decreased ESCC cell proliferation and colony formation. Bioinformatics analysis was used to predict specificity protein 1 (SP1) as a target gene of miR‑375 in ESCC, and this was verified by dual‑luciferase assay. The present study demonstrated that SP1 regulates ESCC cell proliferation and colony formation through direct interaction with miR‑375. In addition, the overall survival of patients with ESCC was analyzed using the Kaplan‑Meier method and log‑rank test. The results indicated that patients with ESCC with high miR‑375 expression had a better survival rate compared with patients with ESCC with low miR‑375 expression. Taken together, these results suggest that downregulated miR‑375 promotes ESCC cell proliferation and colony formation via direct targeting of SP1, and this association may contribute to ESCC progression.

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