Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats

Zheng,Jiahui; Zhang,Shufeng; Wang,Tingting

doi:10.3892/etm.2018.7123

Expression of Notch-1 and nuclear factor-κB signal pathway in myocardial cells of coronary heart disease rats

Authors:
- Jiahui Zheng
- Shufeng Zhang
- Tingting Wang
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Affiliations: Department of Cardiology, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China, Department of Preventive Medicine, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China
Published online on: December 21, 2018 https://doi.org/10.3892/etm.2018.7123
Pages: 1587-1592
Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression changes of Notch-1 and nuclear factor-κB (NF-κB) in cardiomyocytes from coronary heart disease (CHD) rats were investigated. The CHD model established by high-fat diet in 48 clean SD rats was set as the observation group, and another 48 healthy rats routinely fed were the control group. Rats were sacrificed on the 1st, 3rd, 7th, and 14th days after successful modeling. The rat myocardial cells were harvested to examine the changes of Notch-1 and NF-κB using immunoblot (western blot analysis) and TUNEL assay. Cardiomyocyte apoptotic rate, by Pearson's correlation test was used to analyze the correlation between Notch-1 and NF-κB. With the prolongation of the course of CHD in rats, the expression levels of Notch-1 and NF-κB proteins gradually increased, and the expression in the observation group was significantly higher than that in the control group (P<0.01). On the seventh day, the expression levels of Notch-1 and NF-κB protein in the rats in the observation group showed significant difference from those on day 1 and day 3 (P<0.05), and they were significantly different on the 7th and 14th days (P<0.05). There was no correlation between the expression level of the two proteins with the age and sex of the rats. Pearson's correlation analysis showed that Notch-1 was positively correlated with NF-κB protein (r=0.745, P=0.005). The results of myocardial apoptosis test showed that with the prolongation of the CHD course in the rats of the observation group, the cardiomyocyte apoptosis rate did not differ from the control group. The expression of Notch-1 and NF-κB protein is increased in cardiomyocytes of CHD rats. Notch-1 and NF-κB participate in the occurrence and development of CHD.

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