Inhibition of NET‑1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway

  • Authors:
    • Xiangjun Sun
    • Mingchun Wang
    • Fenghua Zhang
    • Xiao Kong
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  • Published online on: January 29, 2019     https://doi.org/10.3892/etm.2019.7211
  • Pages: 2334-2340
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Abstract

The present study aimed to elucidate the underlying mechanism of neuroepithelial cell transforming 1 (NET‑1), a member of the Ras homolog gene family, in hepatocellular carcinoma (HCC). To determine the association between the expression of NET‑1 and the proliferation and migration of MHCC97‑H cells, the cells were transfected with NET‑1 small interfering (si)RNA and si negative control. Following transfection with NET‑1 siRNA, the proliferation rate of MHCC97‑H cells decreased significantly and the percentage of apoptotic cells increased. The HCC cell line MHCC97‑H was used in the present study as it exhibited an increased expression level of NET‑1 compared with the MHCC97‑L cell line. Expression levels of apoptosis‑associated proteins including apoptosis regulator Bax (Bax), cyclinD1, apoptosis regulator Bcl‑2 (Bcl‑2) and caspase‑3 were determined. Expression levels of phosphoinositide 3‑kinase (PI3K) and protein kinase B (AKT) and their phosphorylated forms were also measured by western blotting. Following NET‑1 knockdown, the expression of Bax and cyclinD1 decreased, the expression of Bcl‑2 and caspase‑3 increased, and the PI3K/AKT signaling pathway was inhibited. The results of the present study suggest that inhibition of NET‑1 can suppress the progression of HCC by targeting the PI3K/AKT signaling pathway. NET‑1 expression level in HCC cells increased compared with normal liver cells.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Sun X, Wang M, Zhang F and Kong X: Inhibition of NET‑1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Exp Ther Med 17: 2334-2340, 2019.
APA
Sun, X., Wang, M., Zhang, F., & Kong, X. (2019). Inhibition of NET‑1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway. Experimental and Therapeutic Medicine, 17, 2334-2340. https://doi.org/10.3892/etm.2019.7211
MLA
Sun, X., Wang, M., Zhang, F., Kong, X."Inhibition of NET‑1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway". Experimental and Therapeutic Medicine 17.3 (2019): 2334-2340.
Chicago
Sun, X., Wang, M., Zhang, F., Kong, X."Inhibition of NET‑1 suppresses proliferation and promotes apoptosis of hepatocellular carcinoma cells by activating the PI3K/AKT signaling pathway". Experimental and Therapeutic Medicine 17, no. 3 (2019): 2334-2340. https://doi.org/10.3892/etm.2019.7211