Searching for perturbed biological pathways and genes through analyzing the expression profile changes in osteoclasts after treatment by bisphosphonates

Zhao,Long; Zhang,Bin; Wu,Feng; Chen,Xuan-Huang

doi:10.3892/etm.2019.7219

Searching for perturbed biological pathways and genes through analyzing the expression profile changes in osteoclasts after treatment by bisphosphonates

Authors:
- Long Zhao
- Bin Zhang
- Feng Wu
- Xuan-Huang Chen
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Affiliations: Department of Orthopedics, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, P.R. China, Department of Orthopedics, Chinese Medicine Hospital of Xi'an City, Xi'an, Shanxi 710021, P.R. China, Department of Rehabilitation Medicine, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China, Department of Orthopedics, The Affiliated Hospital of Putian University, Putian, Fujian 351100, P.R. China
Published online on: January 30, 2019 https://doi.org/10.3892/etm.2019.7219
Pages: 2541-2546
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Criticality pathways and genes related to osteoporosis were identified. We downloaded the expression data of osteoclasts treated with or without bisphosphonates and all human pathways from the public database. Gibbs sampling and Markov chain were performed to identify the disturbed pathways and the hub genes in the disturbed pathways. Pathways and genes with adjusted probability (αadj) ≥0.75 were considered as the disturbed pathways and hub genes. We identified four disturbed pathways (Maturity onset diabetes of the young, Olfactory transduction, Cyanoamino acid metabolism, Taurine and hypotaurine metabolism) and two hub genes (OR2A4 and NKX2-2) with αadj ≥0.75. The expression levels of these disturbed pathways and hub genes were downregulated in bisphosphonates group. In conclusion, four disturbed pathways and two hub genes related to osteoporosis were identified. These results give us a better understanding of the potential mechanism of bisphosphonate treatment and the pathogenesis of osteoporosis.

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