MicroRNA‑382 inhibits cancer cell growth and metastasis in NSCLC via targeting LMO3

Chen,Dingzhu; Zhang,Yi; Lin,Yong; Shen,Feimin; Zhang,Zhijian; Zhou,Jiguang

doi:10.3892/etm.2019.7271

MicroRNA‑382 inhibits cancer cell growth and metastasis in NSCLC via targeting LMO3

Authors:
- Dingzhu Chen
- Yi Zhang
- Yong Lin
- Feimin Shen
- Zhijian Zhang
- Jiguang Zhou
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Affiliations: Department of Cardiac and Thoracic Surgery, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China, Department of Clinical Laboratory, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China, Department of Hospital Information, Zhangzhou Hospital Affiliated to Fujian Medical University, Zhangzhou, Fujian 363000, P.R. China
Published online on: February 13, 2019 https://doi.org/10.3892/etm.2019.7271
Pages: 2417-2424
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent studies have revealed a pivotal role of microRNAs (miRs) in regulating the initiation and development of multiple types of cancer. In the present study, it was discovered that miR‑382 may be an important tumor suppressor in non‑small cell lung cancer (NSCLC). It was demonstrated that miR‑382 expression was downregulated in tumor tissues from patients with NSCLC compared with adjacent normal tissues. Furthermore, overexpression of miR‑382 suppressed cell proliferation and cell migration of NSCLC cells. In addition, reverse transcription‑quantitative polymerase chain reaction and the luciferase reporter assay revealed that LIM‑only protein 3 (LMO3), an oncogene, acted as a direct target gene of miR‑382. Notably, overexpression of miR‑382 did not alter cell proliferation or migration in LMO3‑silenced A549 cells. Furthermore, analysis of patient tissues indicated an elevation of LMO3 expression in tumor tissues compared with adjacent normal tissues and a negative association between miR‑382 and LMO3 mRNA expression levels. Taken together, the present findings indicated that miR‑382 inhibited NSCLC cell proliferation and metastasis by targeting LMO3, suggesting a tumor suppressor role of miR‑382 in NSCLC.

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