Matrine inhibits the proliferation of pituitary tumor cells by decreasing Foxo3a phosphorylation and promoting Foxo3a nuclear localization

Li,Luo; Chen,Shuangcheng; Sun,Yunwei; Kong,Lu; Liu,Guangping; Guo,Pin

doi:10.3892/etm.2019.7365

Matrine inhibits the proliferation of pituitary tumor cells by decreasing Foxo3a phosphorylation and promoting Foxo3a nuclear localization

Authors:
- Luo Li
- Shuangcheng Chen
- Yunwei Sun
- Lu Kong
- Guangping Liu
- Pin Guo
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Affiliations: Department of Neurosurgery, Qingdao Municipal Hospital, Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Neurosurgery, RenMin Hospital of Jimo, Qingdao, Shandong 266200, P.R. China, Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China
Published online on: March 8, 2019 https://doi.org/10.3892/etm.2019.7365
Pages: 3775-3780

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Abstract

Pituitary tumors account for 10% of intracranial cancer, and are difficult to treat with chemotherapy. The aim of the present study was to explore the role of matrine on the proliferation of pituitary cancer cells, as well as the molecular mechanism of matrine in progression and development of pituitary tumors. Matrine significantly suppressed the proliferation of pituitary cancer cells in a dose‑ and time‑dependent manner. Western blotting results showed that the phosphorylation levels of AKT serine/threonine kinase (Akt) and forkhead box O3A (Foxo3a) decreased as the concentration of matrine increased. Matrine increased the nuclear localization of Foxo3a and the expression of proapoptotic genes, such as BCL2 like 11 and BCL2 associated X apoptosis regulator, and inhibited the levels of cytoplasmic Foxo3a. In conclusion, matrine promoted cell death of pituitary cancer cells and was involved in Akt/Foxo3a signaling pathway.

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