miR‑758‑3p suppresses human bladder cancer cell proliferation, migration and invasion by targeting NOTCH2

Wu,Xiaofei; Chen,Bin; Shi,Hongbo; Zhou,Ji; Zhou,Fei; Cao,Jun; Sun,Xiaosong

doi:10.3892/etm.2019.7400

miR‑758‑3p suppresses human bladder cancer cell proliferation, migration and invasion by targeting NOTCH2

Authors:
- Xiaofei Wu
- Bin Chen
- Hongbo Shi
- Ji Zhou
- Fei Zhou
- Jun Cao
- Xiaosong Sun
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Affiliations: Department of Urology Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei College of Arts and Sciences, Xiangyang, Hubei 441021, P.R. China
Published online on: March 18, 2019 https://doi.org/10.3892/etm.2019.7400
Pages: 4273-4278

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Abstract

MicroRNAs (miRs) are widely involved in regulating tumor development and progression. miR‑758‑3p has been reported to suppress the progression of various cancer types, including hepatocellular carcinoma. However, whether miR‑758‑3p has a role in bladder cancer (BC) has not been previously reported, and was thus investigated in the present study. It was revealed that miR‑758‑3p was downregulated in BC tissues and cell lines. Transfection with miR‑758‑3p mimics suppressed the proliferation, migration and invasion of BC cells, and inhibition of miR‑758‑3p had the opposite effect. In terms of the underlying mechanisms, a luciferase reporter assay revealed that Notch receptor 2 (NOTCH2) is a direct target gene of miR‑758‑3p in BC cells. Transfection with miR‑758‑3p mimics decreased the mRNA and protein levels of NOTCH2. Furthermore, an inverse correlation between miR‑758‑3p and NOTCH2 levels was identified. Finally, overexpression of NOTCH2 significantly rescued the proliferation, migration and invasion of BC cells transfected with miR‑758‑3p mimics. Taken together, the present study indicated that miR‑758‑3p suppresses BC cell proliferation, migration and invasion by targeting NOTCH2.

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