Expression of miR‑181a and TGF‑β2 in lens epithelial cells of patients with cataractous retinal detachment and its clinical significance
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- Published online on: April 16, 2019 https://doi.org/10.3892/etm.2019.7488
- Pages: 4435-4440
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Abstract
Expression and clinical significance of miR‑181a and TGF‑β2 in lens epithelial cells of patients with cataract‑induced retinal detachment were investigated. Forty patients with rhegmatogenous retinal detachment combined with age‑related cataract (cast‑off group) and another 40 patients with simple age‑related cataract (non‑exfoliated group) in Tongren Hospital were enrolled between January 2017 and June 2018. Lens epithelial cells were collected, and expression levels of miR‑181a and TGF‑β2 mRNA in lens epithelial cells were measured by RT‑qPCR. Expression of protein was detected by western blot analysis. miR‑181a overexpression vector (miR‑181a‑mimic group) was constructed and transfected into lens epithelial cells isolated from patients with rhegmatogenous retinal detachment combined with age‑related cataract. Empty vector (miR‑control group) transfection was performed. Untransfected lens epithelial cells were the control group. Independent sample t-test was performed for comparison between groups. Correlation between miR‑181a and TGF‑β2 protein expression levels was analyzed by Pearson's correlation analysis. Relative expression level of miR‑181a in the non‑exfoliated group was significantly higher than that in the cast‑off group (P<0.05). Relative expression levels of TGF‑β2 mRNA and TGF‑β2 protein in the cast‑off group were significantly higher than those in the non‑exfoliated group (P<0.05). Relative expression level of E‑cadherin in the miR‑control group was significantly lower than that in the miR‑181a‑mimic group (P<0.05). Relative expression level of vimentin in the miR‑control group was significantly higher than that in the miR‑181a‑mimic group (P<0.05). Relative expression level of TGF‑β2 protein in the miR‑control group was significantly higher than that in the miR‑181a‑mimic group (P<0.05). The expression level of miR‑181a was negatively correlated with the expression level of TGF‑β2 protein (r= ‑0.875, P<0.001). miR‑181a expression is decreased, while TGF‑β2 expression is increased in lens epithelial cells of patients with rhegmatogenous retinal detachment combined with age‑related cataract. Over‑expression of miR‑181a may inhibit epithelial cell epithelial‑inhibition by inhibiting TGF‑β2 expression. Our findings may provide guidance for future postoperative prevention and treatment of rhegmatogenous retinal detachment in cataract patients.
