miRNA‑344b‑1‑3p modulates the autophagy of NR8383 cells during Aspergillus fumigatus infection via TLR2

Wu,Yuting; Xu,Hong; Li,Yongqiang; Huang,Dongwei; Chen,Lu; Hu,Yahui; Li,Li; Zhang,Deming; Huang,Wenjie

doi:10.3892/etm.2019.7569

miRNA‑344b‑1‑3p modulates the autophagy of NR8383 cells during Aspergillus fumigatus infection via TLR2

Authors:
- Yuting Wu
- Hong Xu
- Yongqiang Li
- Dongwei Huang
- Lu Chen
- Yahui Hu
- Li Li
- Deming Zhang
- Wenjie Huang
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Affiliations: Department of Geriatric Respiratory Medicine, General Hospital of Southern Theater Command, PLA, Guangzhou, Guangdong 510010, P.R. China, Respiratory Center of PLA, General Hospital of Southern Theater Command, PLA, Guangzhou, Guangdong 510010, P.R. China
Published online on: May 10, 2019 https://doi.org/10.3892/etm.2019.7569
Pages: 139-146

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Abstract

Autophagy serves a pivotal role in host defense during fungal infections, and the contribution by toll‑like receptor 2 (TLR2) has been well demonstrated. It has been reported that microRNA‑344a‑1‑3p (miR‑344a‑1‑3p) can directly target TLR2. However, the expression of TLR2 significantly decreases during Aspergillus fumigatus infection. Therefore, the specific role of miR‑344a‑1‑3p in the host defense against A. fumigatus infection remains to be elucidated. In the present study, A. fumigatus infection increased the expression of TLR2 and induced autophagy, which was indicated by increasing expression levels of autophagy‑related protein 5 (ATG5), Beclin‑1, light chain 3 (LC3)‑1 and LC3‑II, as measured by western blot analysis, and an increased number of GFP‑LC3 puncta, as measured by fluorescence. Following transfection with miR‑344a‑1‑3p mimics and/or TLR2, miR‑344b‑1‑3p significantly inhibited the expression of TLR2, Beclin‑1, ATG5, LC3‑I and LC3‑II, whereas the overexpression of TLR2 significantly attenuated the inhibitory effect on autophagy by miR‑344b‑1‑3p. Collectively, these findings demonstrate that A. fumigatus can be controlled by the induction of autophagy following de‑repression of the expression of TLR2, mediated by miR‑344a‑1‑3p.

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