Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1

Yin,Ruoxi; Chen,Xiaoya

doi:10.3892/etm.2019.7584

Regulatory effect of miR‑144‑3p on the function of human trabecular meshwork cells and fibronectin‑1

Authors:
- Ruoxi Yin
- Xiaoya Chen
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Affiliations: Postgraduate School, Xuzhou Medical University, Xuzhou Eye Hospital, Xuzhou, Jiangsu 221000, P.R. China, Department of Ophthalmology, Xuzhou No. 1 People's Hospital, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical University, Xuzhou Eye Hospital, Xuzhou, Jiangsu 221000, P.R. China
Published online on: May 15, 2019 https://doi.org/10.3892/etm.2019.7584
Pages: 647-653
Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Role of microRNA (miR)‑144‑3p in regulating the function of human trabecular meshwork cells (HTMCs) and fibronectin‑1 (FN‑1) was investigated. HTM cell lines were divided into five groups, of which four groups established oxidative stress HTMC models and one served as a control group. The four model groups were transfected with miR‑144‑3p independent sequence, inhibitory sequence, over‑expression sequence, and the blank group received no transfection. In addition, 40 primary open angle glaucoma patients treated in Xuzhou No. 1 People's Hospital were included in the observation group, and 40 healthy individuals were enrolled as a normal group. RT‑qPCR was used for the detection of miR‑144‑3p expression in serum and cells of patients and healthy people in each group, western blot analysis for FN‑1 expression in cells, CCK‑8 kit for cell proliferation, and Transwell for cell invasion. The expression of serum miR‑144‑3p in the observation group was significantly lower than that in the normal group (P<0.05). The cell optical density value in the over‑expression group was significantly higher than that in the other groups (P<0.05), and in the inhibition group was significantly lower than that in the other groups (P<0.05). The number of cell‑penetration in the over‑expression group was significantly higher than that in the other groups (P<0.05). The expression of FN‑1 protein in the over‑expression group was significantly lower than that in the other four groups (P<0.05), and the expression in the inhibition group was significantly higher than that in the other four groups (P<0.05). The over‑expression of miR‑144‑3p promotes proliferation and invasion of HTMCs by inhibiting the expression of FN‑1 in inoxidative stress HTMCs, and is a potential target for glaucoma treatment.

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