Anti‑inflammatory effects of ethanol extract from the leaves and shoots of Cedrela odorata L. in cytokine‑stimulated keratinocytes

Lee,Han‑Sol; Park,Ji‑Won; Kwon,Ok‑Kyoung; Lim,Yourim; Kim,Jung  Hee; Kim,Soo‑Yong; Zamora,Nelson; Rosales,Kattia; Choi,Sangho; Oh,Sei‑Ryang; Ahn,Kyung‑Seop

doi:10.3892/etm.2019.7639

Anti‑inflammatory effects of ethanol extract from the leaves and shoots of Cedrela odorata L. in cytokine‑stimulated keratinocytes

Authors:
- Han‑Sol Lee
- Ji‑Won Park
- Ok‑Kyoung Kwon
- Yourim Lim
- Jung Hee Kim
- Soo‑Yong Kim
- Nelson Zamora
- Kattia Rosales
- Sangho Choi
- Sei‑Ryang Oh
- Kyung‑Seop Ahn
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Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheonju, Chungcheongbuk 28116, Republic of Korea , International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea, Bioprospecting Research Unit, National Biodiversity Institute, Santo Domingo, Heredia 22‑3100, Costa Rica
Published online on: June 3, 2019 https://doi.org/10.3892/etm.2019.7639
Pages: 833-840
Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cedrela odorata L. is a native plant of the Amazon region. The bark is used in folk remedies for the treatment of diarrhea, vomiting, fever and inflammation. Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease accompanied by itching. It is a complex disease involving environmental factors and genetic factors. In the present study, the anti‑inflammatory and anti‑allergic effects of C. odorata L. methanol extract (COEE) on tumor necrosis factor (TNF)‑α and interferon (IFN)‑γ‑stimulated HaCaT keratinocyte cells were investigated. ELISA and RT‑PCR analysis revealed that the extract had anti‑inflammatory effects, and reduced the interleukin (IL)‑6 and IL‑8 levels of the HaCaT cells. In addition, COEE exhibited anti‑allergic effects, comprising a reduction in the thymus and activation‑regulated chemokine and macrophage‑derived chemokine levels. In addition, pathway analysis and comparison with Bay11‑7082 indicated that these effects are due to the inhibition of nuclear factor (NF)‑κB in TNF‑α/IFN‑γ‑induced HaCaT cells. Therefore, the results of the present study suggest that COEE has anti‑inflammatory and anti‑allergic properties in TNF‑α and IFN‑γ‑stimulated HaCaT cells, which are associated with the inhibition of pro‑inflammatory cytokines and chemokines via the NF‑κB pathway.

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