MicroRNA‑6852 suppresses glioma A172 cell proliferation and invasion by targeting LEF1

Wang,Jialiang; Liu,Haipeng; Zheng,Kebin; Zhang,Shuai; Dong,Wei

doi:10.3892/etm.2019.7762

MicroRNA‑6852 suppresses glioma A172 cell proliferation and invasion by targeting LEF1

Authors:
- Jialiang Wang
- Haipeng Liu
- Kebin Zheng
- Shuai Zhang
- Wei Dong
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Affiliations: Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China
Published online on: July 9, 2019 https://doi.org/10.3892/etm.2019.7762
Pages: 1877-1883

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Abstract

microRNA (miR)‑6852 has been demonstrated to suppress the progression of gastric, colorectal and cervical cancer. The mechanism by which miR‑6852 regulates glioma cells is yet to be elucidated. In the present study, reverse transcription‑quantitative PCR analysis was used and the results demonstrated that miR‑6852 expression was reduced in glioma tissues and cells. Cell counting kit‑8 and transwell assay analysis indicated that proliferation, migration and invasion of A172 cells in the miR‑6852 mimic group were lower than in the miR‑NC group. Compared with the Inh‑NC group, A172 cells of the Inh‑miR‑6852 group exhibited higher proliferation, migration and invasion. Additionally, the results indicated that lymphoid enhancer binding factor 1 (LEF1) was directly inhibited by miR‑6852 and LEF1 expression was negatively correlated with miR‑6852 expression in glioma tissues. Furthermore, the restoration of LEF1 reversed the effects of the miR‑6852 mimics. The present findings suggested that miR‑6852 inhibited glioma cells proliferation, migration and invasion by targeting the suppression of LEF1.

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