Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE

Liu,Hongwei; Zhang,Jian; Zhou,Pingping; Sun,Hao; Katsarou,Martha; Drakoulis,Nikolaos

doi:10.3892/etm.2019.8009

Exploration of vascular adhesion protein-1 expression in patients with conjunctivitis associated systemic lupus erythematosus using 2D-DIGE

Authors:
- Hongwei Liu
- Jian Zhang
- Pingping Zhou
- Hao Sun
- Martha Katsarou
- Nikolaos Drakoulis
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Affiliations: Department of Ophthalmology, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154003, P.R. China, Department of Ophthalmology, The Huai'an Affiliated Hospital of Xuzhou Medical University, Huai'an, Jiangsu 223002, P.R. China, Research Group of Clinical Pharmacology and Pharmacogenomics, Faculty of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, 15771 Athens, Greece
Published online on: September 16, 2019 https://doi.org/10.3892/etm.2019.8009
Pages: 5072-5077

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Abstract

Conjunctivitis associated systemic lupus erythematosus (caSLE) is a connective tissue autoimmune disease with a large spectrum of clinical manifestations. The disease may lead to ocular complications and in severe cases may be sight‑threatening. This study investigated the expression of vascular adhesion protein‑1 (VAP‑1) in SLE patients with conjunctivitis and the relationship between VAP‑1 and other proteins in the onset of the disease. Ten patients with caSLE (caSLE group) and 10 healthy volunteers (control group) were enrolled in the study, in order to evaluate the VAP‑1 expression levels in blood. Protein expression profiling was performed with two‑dimensional fluorescence difference gel electrophoresis (2D‑DIGE) and analyzed with matrix‑assisted laser desorption/deionization time‑of‑flight (MALDI‑TOF) used in tandem with mass spectrometry. In the caSLE group, 8 proteins were expressed differenty compared with the control group: C‑reactive protein, hemoglobin subunit β, VAP‑1, A‑albumin (AFAM), enolase and immunoglobulin heavy constant mu were upregulated; interferon regulatory factor‑1 and serum amyloid A2 protein were downregulated. Western blotting results are consistent with the proteomics results, showing that in caSLE group VAP‑1 expression is increased in comparison to the control group. VAP‑1 protein participates in the inflammatory reaction in the form of amine oxidase, copper containing 3. Bioinformatics analysis suggested that VAP‑1 protein could be used as a candidate protein for further study regarding its role as a potential protein marker for screening and effectively monitoring caSLE.

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