Inhibition of miRNA‑34a promotes triple negative cancer cell proliferation by promoting glucose uptake

Li,Zhen; Gong,Xiaoxuan; Zhang,Wei; Zhang,Jian; Ding,Li; Li,Hao; Tu,Daoyuan; Tang,Jinhai

doi:10.3892/etm.2019.8017

Inhibition of miRNA‑34a promotes triple negative cancer cell proliferation by promoting glucose uptake

Authors:
- Zhen Li
- Xiaoxuan Gong
- Wei Zhang
- Jian Zhang
- Li Ding
- Hao Li
- Daoyuan Tu
- Jinhai Tang
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Affiliations: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Published online on: September 18, 2019 https://doi.org/10.3892/etm.2019.8017
Pages: 3936-3942
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

miRNA‑34a is a tumor suppressor that is expressed in a variety of different types of cancer. The current study aimed to determine the involvement of miRNA‑34a in triple negative breast cancer. miRNA‑34a expression was detected using reverse transcription‑quantitative PCR in the breast tissue and serum of patients with triple negative breast cancer and of healthy controls. The diagnostic value of miRNA‑34a in triple negative breast cancer was analyzed using receiver operating curve analysis. A miRNA‑34a inhibitor was transfected into triple negative breast cancer cells and the effects on cell proliferation, glucose uptake and glucose transporter 1 (GLUT1) expression were detected using a cell counting kit‑8 assay, glucose uptake assay and western blot analysis, respectively. The results demonstrated that miRNA‑34a was downregulated in patients with triple negative breast cancer compared with healthy controls and the downregulation of miRNA‑34a effectively distinguished patients with triple negative breast cancer from healthy controls. miRNA‑34a inhibition promoted cancer cell proliferation, accelerated glucose uptake and upregulated GLUT1. The current study concluded that the inhibition of miR‑34a may promote triple negative cancer cell proliferation by promoting glucose uptake.

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