MicroRNA‑139‑5p regulates chronic inflammation by suppressing nuclear factor‑κB activity to inhibit cell proliferation and invasion in colorectal cancer

Zhu,Mingming; Zhang,Wen; Ma,Jun; Dai,Youguo; Zhang,Qi; Liu,Qin; Yang,Burong; Li,Gang

doi:10.3892/etm.2019.8032

MicroRNA‑139‑5p regulates chronic inflammation by suppressing nuclear factor‑κB activity to inhibit cell proliferation and invasion in colorectal cancer

Authors:
- Mingming Zhu
- Wen Zhang
- Jun Ma
- Youguo Dai
- Qi Zhang
- Qin Liu
- Burong Yang
- Gang Li
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Affiliations: Department of Abdominal Tumor Surgery, Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650118, P.R. China
Published online on: September 20, 2019 https://doi.org/10.3892/etm.2019.8032
Pages: 4049-4057
Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The inﬂammatory microenvironment, which mediates the initiation and malignant development of tumors, has been reported to be associated with microRNA (miRNA) dysregulation. In the present study, the expression of miR‑139‑5p was analyzed in colorectal cancer (CRC) cell lines SW480, HT29, HCT‑8, LoVo and HCT116, aiming to investigate the function and mechanism of miR‑139‑5p in the regulation of the malignant phenotypes of CRC. miR‑139‑5p expression was found to be considerably downregulated in CRC cell lines compared with the human normal colon mucosal epithelial cell line NCM460. Subsequently, it was demonstrated that overexpression of miR‑139‑5p in colon cancer cell lines significantly suppressed the cell proliferation in vitro and in vivo. In addition, overexpression of miR‑139‑5p further inhibited the invasion ability of colon cancer cells in vitro, concomitantly with downregulation of key invasion‑associated proteins, including matrix metalloproteinase 9 (MMP9) and MMP7. Furthermore, it was demonstrated that overexpression of miR‑139‑5p decreased the expression levels of inﬂammatory cytokines, including interleukin‑1β (IL‑1β), IL‑6 and tumor necrosis factor‑α (TNF‑α), by suppressing nuclear factor (NF)‑κB activity. Therefore, these findings collectively indicated that miR‑139‑5p regulated chronic inflammation by suppressing NF‑κB activity in order to inhibit cell proliferation and invasion in CRC, thereby indicating a novel molecular mechanism in CRC therapy.

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