Effect of microRNA‑133a‑3p/matrix metalloproteinase‑9 axis on the growth of atherosclerotic vascular smooth muscle cells

Shi,Lei; Yu,Chunpeng; Tian,Xintao; Ma,Chengtai; Wang,Lumin; Xia,Di; Cui,Changxing; Chen,Xiaoxue; Jiang,Tao; Gu,Yan; Liu,Zhenfang; Cai,Shanglang

doi:10.3892/etm.2019.8070

Effect of microRNA‑133a‑3p/matrix metalloproteinase‑9 axis on the growth of atherosclerotic vascular smooth muscle cells

Authors:
- Lei Shi
- Chunpeng Yu
- Xintao Tian
- Chengtai Ma
- Lumin Wang
- Di Xia
- Changxing Cui
- Xiaoxue Chen
- Tao Jiang
- Yan Gu
- Zhenfang Liu
- Shanglang Cai
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Affiliations: Department of Emergency Internal Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266071, P.R. China, Department of Interventional Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266071, P.R. China, Department of Cardiovascular Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China
Published online on: October 1, 2019 https://doi.org/10.3892/etm.2019.8070
Pages: 4356-4362
Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Atherosclerosis (AS) is the leading cause of cardiovascular disease and poses a threat to human health. MicroRNAs (miRNAs/miRs) are a group of endogenous small non‑coding RNAs that have been identified to serve important roles in AS. However, the expression and role of miR‑133a‑3p in AS remains unclear. The aim of the present study was to investigate miR‑133a‑3p in AS and to determine its underlying mechanism. The level of miR‑133a‑3p expression in the blood and vascular plaque tissue of patients with AS was detected via reverse transcription‑quantitative PCR (RT‑qPCR). The role of miR‑133a‑3p in human vascular smooth muscle cells (hVSMCs) was investigated, following upregulation and downregulation of this miR in hVSMCs. Cell proliferation and apoptosis were determined using a Cell Counting kit‑8 assay and flow cytometry, respectively. The results demonstrated the downregulation of miR‑133a‑3p in the blood and vascular plaque tissue of patients with AS. Matrix metallopeptidase‑9 (MMP‑9) was revealed to be a direct target gene of miR‑133a‑3p, which was upregulated in the blood and vascular plaque tissue of patients with AS. Furthermore, MMP‑9 was determined to be negatively regulated by miR‑133a‑3p in hVSMCs. In addition, significant inhibition of hVSMC proliferation and induction of cell apoptosis were observed following MMP‑9 downregulation and following transfection with the miR‑133a‑3p mimic. The effects of the miR‑133a‑3p mimic on hVSMC proliferation and apoptosis were reversed by MMP‑9 over‑expression. Overall, the results indicated that miR‑133a‑3p was downregulated in AS, which results in the inhibition of hVSMC proliferation and the induction of cell apoptosis via MMP‑9. miR‑133a‑3p may therefore be a promising therapeutic target for the treatment of AS.

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