LncRNA AWPPH participates in the development of non‑traumatic osteonecrosis of femoral head by upregulating Runx2

Chen,Xiantao; Li,Jing; Liang,Dawei; Zhang,Leilei; Wang,Qingfeng

doi:10.3892/etm.2019.8185

LncRNA AWPPH participates in the development of non‑traumatic osteonecrosis of femoral head by upregulating Runx2

Authors:
- Xiantao Chen
- Jing Li
- Dawei Liang
- Leilei Zhang
- Qingfeng Wang
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Affiliations: Department of Osteonecrosis of The Femoral Head, Luoyang Orthopedic Hospital of Henan Province, Luoyang, Henan 471000, P.R. China
Published online on: November 12, 2019 https://doi.org/10.3892/etm.2019.8185
Pages: 153-159
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

AWPPH is a newly discovered long noncoding (lnc)RNA that plays an oncogenic role in development of several types of malignancies, whiles its involvement in non‑traumatic osteonecrosis of femoral head (ONFH) is unknown. Therefore, the present study aimed to investigate the functionality of AWPPH in non‑traumatic ONFH. Blood and mesenchymal stem cells (MSCs) were obtained from both non‑traumatic ONFH patients and healthy controls, and expression of AWPPH in those tissues was detected by RT‑qPCR. Receiver operating characteristic curve analysis was performed to investigate the diagnostic value of lncRNA AWPPH expression for non‑traumatic ONFH. Bone morphogenic protein (BMP‑2) was used to treat MSCs to induce osteogenic differentiation and the effects on lncRNA AWPPH expression was detected by RT‑qPCR. LncRNA AWPPH overexpression and short hairpin (sh)RNA silencing cell lines were established and the effects on runt‑related transcription factor 2 (Runx2) expression were detected by western blotting. It was demonstrated that AWPPH was significantly downregulated in non‑traumatic ONFH patients compared with in healthy controls in both MSCs and serum. Expression of AWPPH in MSCs and serum is a sensitive diagnostic marker for non‑traumatic ONFH. Expression of AWPPH exhibited no significant correlation with patients' age, gender and living habits, but was significantly correlated with course of disease. BMP‑2 treatment significantly increased the expression level of AWPPH in human MSCs from bone marrow (hMSC‑BM). AWPPH overexpression promoted, while AWPPH short hairpin RNA silencing inhibited the expression of Runx2 expression in hMSC‑BM cells. Therefore, it was concluded that lncRNA AWPPH may participate in the development of ONFH by upregulating Runx2.

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