Matrine suppresses breast cancer metastasis by targeting ITGB1 and inhibiting epithelial‑to‑mesenchymal transition

Ren,Lili; Mo,Wenju; Wang,Linling; Wang,Xiaojia

doi:10.3892/etm.2019.8207

Matrine suppresses breast cancer metastasis by targeting ITGB1 and inhibiting epithelial‑to‑mesenchymal transition

Authors:
- Lili Ren
- Wenju Mo
- Linling Wang
- Xiaojia Wang
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Affiliations: Department of Integration of Traditional Chinese and Western Medicine, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Department of Breast Tumor Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
Published online on: November 18, 2019 https://doi.org/10.3892/etm.2019.8207
Pages: 367-374
Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Metastasis can be a fatal step in breast cancer progression. Effective therapies are urgently required due to the limited therapeutic options clinically available. The aim of the present study was to investigate the effect of matrine (MAT), a traditional Chinese medicine, on the proliferation and migration of human breast cancer cells and its underlying mechanisms of action. The proliferation of MDA‑MB‑231 cells was inhibited and apoptosis was induced following treatment with MAT, as determined by MTT and Annexin‑V‑FITC/PI assays. Western blot analysis was used to detect the LC‑3II/I levels and the results suggested that tumor autophagy is involved in the anti‑tumor activity of MAT. To the best of our knowledge, this is the first study to report that MAT inhibits MDA‑MB‑231 and MCF‑7 cell motility, potentially by targeting integrin β1 (ITGB1) and epithelial‑to‑mesenchymal transition (EMT), as indicated by Transwell® and siRNA interference assays. In conclusion, ITGB1 and EMT are involved in MAT‑induced breast carcinoma cell death and the inhibition of metastasis. This may lead to the development of novel compounds for the treatment of breast cancer metastasis.

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