miR‑770‑5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1

  • Authors:
    • Dawei Zhang
    • Ying Li
    • Peilong Sun
  • View Affiliations

  • Published online on: November 19, 2019     https://doi.org/10.3892/etm.2019.8221
  • Pages: 339-346
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colon cancer is one of the most common types of cancer worldwide. Methotrexate (MTX) is a chemotherapy drug used for the treatment of multiple types of cancer, such as colon and breast cancer. To determine the effects of MTX treatment on colorectal adenocarcinoma cell lines, a microRNA (miRNA) microarray was used to detect miRNA expression profiles of HT‑29 colorectal adenocarcinoma MTX‑resistant cells and their parental cells. The results demonstrated that 641 genes and 43 miRNAs were differentially expressed between HT‑29 MTX‑sensitive cells and MTX‑resistant cells. In addition, 12 miRNAs and their co‑expressed genes were highly correlated in MTX treatment, and one of the identified miRNAs, miR‑770‑5p, was studied in subsequent experiments. Upregulation of miR‑770‑5p significantly decreased the sensitivity of HT‑29 cells to MTX. Using bioinformatics software, homeodomain‑interacting protein kinase 1 (HIPK1) was identified to be a putative target gene of miR‑770‑5p, which was confirmed by a luciferase reporter assay. Downregulation of miR‑770‑5p target gene HIPK1 significantly decreased the sensitivity of HT‑29 cells to MTX. These results suggest that miR‑770‑5p may be involved in the regulation of colon cancer resistance to MTX by regulating the expression of the target gene HIPK1.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang D, Li Y and Sun P: miR‑770‑5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1. Exp Ther Med 19: 339-346, 2020.
APA
Zhang, D., Li, Y., & Sun, P. (2020). miR‑770‑5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1. Experimental and Therapeutic Medicine, 19, 339-346. https://doi.org/10.3892/etm.2019.8221
MLA
Zhang, D., Li, Y., Sun, P."miR‑770‑5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1". Experimental and Therapeutic Medicine 19.1 (2020): 339-346.
Chicago
Zhang, D., Li, Y., Sun, P."miR‑770‑5p modulates resistance to methotrexate in human colorectal adenocarcinoma cells by downregulating HIPK1". Experimental and Therapeutic Medicine 19, no. 1 (2020): 339-346. https://doi.org/10.3892/etm.2019.8221