Edaravone combined with cinepazide maleate on neurocyte autophagy and neurological function in rats with subarachnoid hemorrhage

Cai,Zhenli; Zhang,Haidong; Song,Hongshan; Piao,Ying; Zhang,Xiaoye

doi:10.3892/etm.2019.8240

Edaravone combined with cinepazide maleate on neurocyte autophagy and neurological function in rats with subarachnoid hemorrhage

Authors:
- Zhenli Cai
- Haidong Zhang
- Hongshan Song
- Ying Piao
- Xiaoye Zhang
View Affiliations

Affiliations: Department of Neurology, Daqinglongnan Hospital, Daqing, Heilongjiang 163453, P.R. China, Department of Oncology, Daqing People's Hospital, Daqing, Heilongjiang 163000, P.R. China
Published online on: November 25, 2019 https://doi.org/10.3892/etm.2019.8240
Pages: 646-650
Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Effects of edaravone combined with cinepazide maleate on neurocyte autophagy and neurological function in rats with subarachnoid hemorrhage were investigated. Eighty SD rats were selected to establish subarachnoid hemorrhage (SAH) rat models, which were divided into sham operation group, SAH group, MCI group and combined group. Hippocampal tissue of each group was taken to observe the number of neurocytes. The expression levels of Beclin-1 and (light chain LC3)-II of rats in each group were detected by ELISA. Pearson's correlation factors were used to analyze the correlation between Beclin-1 and LC3-11, and neurological function tests were carried out on rats of each group 14 and 28 days after administration. The morphological and structural damage of nerve cells in the combined group was further alleviated, and the survival rate of neurons significantly increased at all time points (P<0.05). The expression levels of Beclin-1 and LC3-11 in combined group was significantly higher than those in SAH group and CMI group (P<0.05), and Beclin-1 was positively correlated with LC3-11 (r=0.9454). Longa score of the combined group was significantly lower than that of the other two groups, and muscle strength score was significantly higher than that of the other two groups (P<0.05). Edaravone combined with cinepazide maleate can effectively increase the survival rate of brain cells and promote the volatilization of neurological function in the treatment of hemorrhage in the subretinal space of the omentum, which is worthy of popularization and application.

View Figures

View References

1	Feigin VL, Rinkel GJ, Lawes CM, Algra A, Bennett DA, van Gijn J and Anderson CS: Risk factors for subarachnoid hemorrhage: An updated systematic review of epidemiological studies. Stroke. 36:2773–2780. 2005. View Article : Google Scholar : PubMed/NCBI
2	Locksley HB: Natural history of subarachnoid hemorrhage, intracranial aneurysms and arteriovenous malformations. Based on 6368 cases in the cooperative study. J Neurosurg. 25:219–239. 1966. View Article : Google Scholar : PubMed/NCBI
3	Kotwica Z and Persson L: Effect of intravenous infusion of nimodipine on intracranial pressure. Experimental study of ICP measurement conducted in a rat model. Neurol Neurochir Pol. 23:227–230. 1989.(In Polish). PubMed/NCBI
4	Akashi A, Hirohashi M, Suzuki I, Shibamura S and Kasahara A: Cardiovascular pharmacology of cinepazide, a new cerebral vasodilator (author's transl). Nippon Yakurigaku Zasshi. 75:507–516. 1979.(In Japanese). View Article : Google Scholar : PubMed/NCBI
5	Yi ZM, Liu F and Zhai SD: Cinepazide maleate injection for cerebral infarction: a systematic review. Chin J Evidence-Based Med. 10:1079–1084. 2010.
6	Kono H, Asakawa M, Fujii H, Maki A, Amemiya H, Yamamoto M, Matsuda M and Matsumoto Y: Edaravone, a novel free radical scavenger, prevents liver injury and mortality in rats administered endotoxin. J Pharmacol Exp Ther. 307:74–82. 2003. View Article : Google Scholar : PubMed/NCBI
7	Jin Q, Cai Y, Li S, Liu H, Zhou X, Lu C, Gao X, Qian J, Zhang J, Ju S and Li C: Edaravone-encapsulated agonistic micelles rescue ischemic brain tissue by tuning blood-brain barrier permeability. Theranostics. 7:884–898. 2017. View Article : Google Scholar : PubMed/NCBI
8	Wang Z, Shi XY, Yin J, Zuo G, Zhang J and Chen G: Role of autophagy in early brain injury after experimental subarachnoid hemorrhage. J Mol Neurosci. 46:192–202. 2012. View Article : Google Scholar : PubMed/NCBI
9	Lee JY, He Y, Sagher O, Keep R, Hua Y and Xi G: Activated autophagy pathway in experimental subarachnoid hemorrhage. Brain Res. 1287:126–135. 2009. View Article : Google Scholar : PubMed/NCBI
10	Zheng XW, Yang WT, Chen S, Xu QQ, Shan CS, Zheng GQ and Ruan JC: Neuroprotection of catalpol for experimental acute focal ischemic stroke: Preclinical evidence and possible mechanisms of antioxidation, anti-inflammation, and antiapoptosis. Oxid Med Cell Longev. 2017:50586092017. View Article : Google Scholar : PubMed/NCBI
11	Lu XY and Hao XM: Biological effects of electrical stimulation on muscle strength and electromyogram in rats with muscle disuse atrophy. Chin J Clin Rehabil. 10:34–36. 2006.(In Chinese).
12	McGirt MJ, Mavropoulos JC, McGirt LY, Alexander MJ, Friedman AH, Laskowitz DT and Lynch JR: Leukocytosis as an independent risk factor for cerebral vasospasm following aneurysmal subarachnoid hemorrhage. J Neurosurg. 98:1222–1226. 2003. View Article : Google Scholar : PubMed/NCBI
13	Hillbom M and Kaste M: Alcohol intoxication: A risk factor for primary subarachnoid hemorrhage. Neurology. 32:706–711. 1982. View Article : Google Scholar : PubMed/NCBI
14	Yolas C, Ozdemir NG, Kanat A, Aydin MD, Keles P, Kepoglu U, Aydin N and Gundogdu C: Uncovering a new cause of obstructive hydrocephalus following subarachnoid hemorrhage: choroidal artery vasospasm-related ependymal cell degeneration and aqueductal stenosis-first experimental study. World Neurosurg. 90:484–491. 2016. View Article : Google Scholar : PubMed/NCBI
15	Hartmann A, Dorndorf W and Alberti E: Complications of subarachnoid hemorrhage. Med Klin. 72:476–482. 1977.PubMed/NCBI
16	Lee JY, Sagher O, Keep R, Hua Y and Xi G: Comparison of experimental rat models of early brain injury after subarachnoid hemorrhage. Neurosurgery. 65:331–343. 2009. View Article : Google Scholar : PubMed/NCBI
17	Chao Y, Hu W and Geng X: The neuroprotective strategic analysis for patients with acute myocardial infarction combined with hypoxic ischemic encephalopathy in ICU. Hellenic J Cardiol. 58:427–431. 2017. View Article : Google Scholar : PubMed/NCBI
18	Zheng PU, Wang XR, Gan J and Chen QI: Effect of cinepazide maleate preconditioning on rat models of cerebral ischemia. J Shanghai Jiaotong Univ. 33:1064–1073. 2013.(In Chinese).
19	Wang L, Wang P, Dong H, Wang S, Chu H, Yan W and Zhang X: Ulk1/FUNDC1 prevents nerve cells from hypoxia-induced apoptosis by promoting cell autophagy. Neurochem Res. 43:1539–1548. 2018. View Article : Google Scholar : PubMed/NCBI
20	Munakata A, Ohkuma H, Nakano T, Shimamura N, Asano K and Naraoka M: Effect of a free radical scavenger, edaravone, in the treatment of patients with aneurysmal subarachnoid hemorrhage. Neurosurgery. 64:423–429. 2009. View Article : Google Scholar : PubMed/NCBI
21	Wei W, Yang G, Shu XU, Yin W-B and Ding X-S: The brain-protective effects of edaravone in rats after subarachnoid hemorrhage. Chin J Contemp Neurol Neurosurg. 8:544–549. 2008.(In Chinese).
22	Nakagomi T, Yamakawa K, Sasaki T, Saito I and Takakura K: Effect of edaravone on cerebral vasospasm following experimental subarachnoid hemorrhage. J Stroke Cerebrovasc Dis. 12:17–21. 2003. View Article : Google Scholar : PubMed/NCBI