Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway

Yun,Changjun; Qian,Wenjie; Wu,Junyi; Yuan,Chenxi; Jiang,Songzhou; Lv,Jinpeng

doi:10.3892/etm.2019.8286

Pilose antler peptide promotes osteoblast proliferation, differentiation and mineralization via the insulin signaling pathway

Authors:
- Changjun Yun
- Wenjie Qian
- Junyi Wu
- Chenxi Yuan
- Songzhou Jiang
- Jinpeng Lv
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Affiliations: Department of Orthopedics, Wujin Hospital Affiliated with Jiangsu University, Changzhou, Jiangsu 213000, P.R. China, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, Jiangsu 213000, P.R. China
Published online on: December 5, 2019 https://doi.org/10.3892/etm.2019.8286
Pages: 923-930
Copyright: © Yun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoporosis is a severe bone disease characterized by a decrease in the density and structure of bones, with high risks of fractures. Pilose antler peptide (PAP), extracted and purified from deer antlers, can promote regeneration and fracture healing, and strengthen sinews and bone. To determine whether PAP can promote osteoblast development and to elucidate the molecular mechanisms underlying its functions, the present study investigated the effects of PAP on osteoblast proliferation, differentiation and mineralization, and the role of the insulin signaling pathway using MTT assay, alkaline phosphatase activity assay, western blot analysis and reverse transcription‑quantitative PCR. The present results suggested that PAP promoted osteoblast proliferation, differentiation and mineralization in vitro via the insulin signaling pathway. The effect of PAP on insulin signaling in osteoblasts may be mediated via the ERK pathway and partially by the PI3K/Akt pathway. The present results indicated that PAP could potentially be developed as an alternative treatment strategy for bone diseases related to diabetes characterized by insulin signaling impairment.

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