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Investigation of the deposition behaviour and antibacterial effectivity of allicin aerosols and vapour using a lung model
- Authors:
- Published online on: December 27, 2019 https://doi.org/10.3892/etm.2019.8387
- Pages: 1541-1549
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Copyright: © Reiter et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Natural defence compounds, such as the antibiotic substance, allicin (diallylthiosulfinate), are produced by garlic tissues when the cells are damaged (1–3). Allicin can be easily synthesized in the laboratory by an oxidation of diallyl disulphide (DADS) (4). The substrate for allicin biosynthesis is S-allyl-L-cysteine sulfoxide, an odourless non-toxic non-protein amino acid found in the cytosol of garlic cells (5). The enzyme, alliin lyase (alliinase, E.C.4.4.1.4), is present in the cell vacuole and hydrolyses alliin to dehydroalanine and allylsulfenic acid. Two molecules of allylsulfenic acid condense spontaneously to produce one molecule of allicin (Fig. 1).
Allicin crosses cell membranes easily and reacts with thiol groups in glutathione (GSH) and accessible cysteines in proteins to yield S-thioallylmercapto derivatives (6–10). Allicin has been shown to have pronounced antimicrobial activities against multiple drug-resistant (MDR) human lung pathogens (11). The effects of allicin on bacterial proteins have been well documented (10,12) and a recent study identified a series of proteins S-thioallylated by allicin in human cells in culture (13). However, as allicin is rapidly titrated out by GSH and metabolized in the human body, the bioavailability of allicin per se following consumption is generally low (14). Thus, any attempts to use allicin to combat microbial infections encounter the obstacle of achieving therapeutic concentration at the site of infection, particularly if allicin is administered orally or is injected into the blood (15,16). The direct inhalation of allicin vapour or aerosols potentially offers an immediate route for the treatment of lung pathogens which would avoid these drawbacks. Indeed, there is a historical precedent from the pre-streptomycin era reporting a high success rate for treating tuberculosis in patients by inhaling the vapour from crushed garlic pulp held in a specially designed inhaler (17) (Fig. 2). An enlightening summary of these studies has been reported by Block (2). Moreover, allicin has been shown to have antimicrobial activity against several human lung pathogenic bacteria (11). The development of allicin-inspired fluoroquinolones, such as antibacterials against Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp. (ESKAPE) pathogens was also recently reported (18).
The emergence of bacteria exhibiting multiple resistance to antibiotics in current clinical use is an increasing threat to the effective treatment of infectious diseases. Frequently, resistance emerges rapidly following the discovery of an antibiotic, particularly after its introduction into clinical practice (19,20). There have been few new classes of antibiotics discovered since the 1970s and novel antibiotics are desperately required (21,22). MDR strains of lung pathogenic bacteria, such as Pseudomonas aeruginosa, Streptococcus pneumoniae, Staphylococcus aureus, Acinetobacter baumannii and Mycobacterium tuberculosis have been reported and lung infections are becoming increasingly difficult to treat (23–27). New categories of antibiotics with multiple sites of action, such as allicin, are particularly desirable as they are likely to render the emergence of resistance more difficult.
Any novel antibiotic compounds require testing in pre-clinical trials, which out of necessity, involve costly and extensive animal studies. It is desirable to know as much as possible about the behaviour of a test substance before progressing to animal trials and in this regard, in this study, we designed and constructed a test rig that accurately recapitulates the air-flow through the life-size 2nd, 3rd, 4th and 5th bronchi of a human lung in order to help characterize the behaviour of inhaled antibiotics. The detailed construction and aerodynamic characteristics of air-flow in this model have been previously reported (28). Herein, we report that using this model, it is possible to demonstrate the dose-dependent efficacy of gentamicin aerosols, and allicin aerosols and vapour. Moreover, differences in the susceptibility of bacteria to the antibiotics were demonstrated in the model and synergism for the inhibitory effect to bacteria was revealed between ethanol and allicin in the gas phase. Therefore, initial proof of principle for using the lung model to predict the behaviour of antibiotics supplied to the lungs has been shown, and it seems that some clinically relevant questions can be addressed using the test rig prior to clinical trials, which will reduce the need for some animal experiments.
Materials and methods
Allicin synthesis and application
Allicin was synthesized by the oxidation of DADS with H2O2 as described previously (4).
Bacteria
The E. coli strain, MegaX DH10B T1R (Invitrogen/Thermo Fisher Scientific), was transformed with either the empty vector pRU1097 (=allicin-susceptible E. coli strain) or with pRU1097 containing a 9 kb allicin resistance-conferring genomic clone from an allicin-resistant Pseudomonas fluorescens isolated from a garlic bulb (=allicin-resistant E. coli strain) (29). As the pRU1097 vector (30) carries a gentamicin resistance gene as a selectable marker, both transformant lines have high gentamicin resistance.
Lung model
The construction details of the lung model have been previously reported (28). The lung model represents the 2nd to 5th generation (the trachea=0th generation) of average life-size bronchial passages in a human lung (Fig. 3A). The model is approximately 10×10 cm. The inner coating of agar medium simulates the epithelial surface of the bronchi and allows for the incorporation of bacteria to simulate an infected lung. In the agar-coated model, the internal diameter of the initial bronchus is 8.3 mm and the internal diameter of the 5th bronchus is 3.5 mm. The agar-coated bronchi were prepared in two halves, which were subsequently positioned together with the help of locating pins and screw clamps to make the bronchial tubes.
The casting process of the bronchial-surface coating of bacteria-seeded agar was performed using a negative mould with a positive stamp with an offset of 1 mm (Fig. 4). The mould and stamp were turned from blocks of polyoxymethylene (POM) with a milling machine. Before the agar surface was cast, the surfaces were sterilized by UV light under a clean bench (Labgard® Class II NU-437-500E, NuAir) for 20 min. The Luria-Bertani (LB) medium with the addition of 1.5% (w/v) agar (Agar-Agar Kobe I, powdered, for microbiology, Carl Roth) was autoclaved for 20 min at 121°C and subsequently tempered in a water bath at 50°C prior to use. A 300 µl aliquot of bacterial suspension from a log phase culture grown to an optical density of 0.2 at wavelength 600 nm (OD600=0.2) was mixed with a 20 ml aliquot of agar tempered to 50°C and was rapidly filled into the mould. Immediately afterwards, the stamp was pushed down by the precise positioning of dowel pins (Fig. 4). The stamp was held for 5 min to ensure complete agar solidification. Subsequently, the stamp was removed and excess medium at the inlet and outlets was trimmed off with a scalpel to obtain sharp inlet and outlet contours. This procedure was repeated for the other half of the mould before both halves were assembled to yield the model bronchial system.
The assembled agar-coated bronchial passages within the upper and lower mould halves was then placed in the incubation chamber and attached to the aerosol-generator and air supply (Fig. 5). A TurboBOY SX with the PARI LC SPRINT nebuliser nozzle attachment (Pari GmbH) was used to generate the aerosol particles. According to the manufacturer, this set-up produces aerosol particles with the mass median diameter (MMD) of 3.5 µm with mass percentage below 5 µm 67% and total output rate of 600 mg/min (measured with 0.9% NaCl solution and 20 l/min inspiratory flow). The nebulizer was attached to the compressor and external air-supply and pressure gauge. The air-supply was set to 6 l/min and the compressor delivered 5 l/min and this provided in total, 11 l/min air-flow entering the lung model via the long inlet tube with an internal diameter of 8.3 mm (Fig. 5). The inlet tube connected seamlessly with the agar-coated lung model and ensured a uniform laminar air-flow into the model.
The temperature in the incubator chamber was regulated by a copper heating plate with aluminium cooling fins attached and on-off settings at 36.5 and 37.5°C, respectively. The thermostat temperature sensor was located at the upper edge of the long side of the incubator and air was circulated within the chamber by means of a fan placed 1 cm from the heating plate. A 1.5 cm diameter air outlet from the incubation chamber was fitted with a 0.45 µm bacteria filter.
Treatment
For aerosol treatment, allicin or gentamicin solutions (diluted in deionised water) were used. The allicin solutions were prepared from stock solutions and the concentrations were confirmed by high-performance liquid chromatography (HPLC) as previously described (4). For the treatment with allicin vapour, allicin was diluted in 95% ethanol and placed into a shallow plastic vessel to yield a 5 cm2 surface for evaporation at room temperature into the gas phase. Following treatment, the residual volume of allicin solution was measured and the concentration was determined by HPLC. The quantity of allicin entering the lung model over the period of the treatment could be calculated from the residual allicin solution.
Visualization of bacterial growth and growth inhibition
At the end of an experiment, and after the air-flow was discontinued, the lung model assembly was taken out of the incubation chamber and after covering with moist filter paper, was wrapped in aluminium foil and incubated at 37°C for 18 h to allow for bacterial growth. The apparatus was then dismantled and separated, exposing the inner surfaces of the two halves of agar tubes representing the bronchi. The halves were sprayed with 0.5% 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) solution, covered with transparent polyethylene foil and incubated at 37°C for 30 min. Metabolically active bacteria reduced the MTT to dark coloured formazan and where bacteria were inhibited, the agar remained pale.
Results
The detailed modelling of the test rig has been previously described (28)
The qualitative axial velocity distribution through the bronchi is shown as a spectral heatmap in Fig. 6. High air-flow velocities are at the red end of the visible colour spectrum and low air-flow velocities at the blue end. The highest velocities were at the inner surface of the carinal surfaces after the branch points. Low velocity ‘shadow areas’ are also apparent, especially at the outer edge at the first dichotomy.
Visualization of bacterial growth
A non-pathogenic E. coli strain (MegaX DH10B™ T1R transformed with empty pRU1097 vector) was used for the characterization of the behaviour of the test rig in the experiments reported in this study, as research with human pathogens demands particularly stringent containment conditions in specialized facilities. The feasibility of showing bacterial growth and growth inhibition within the bronchi of the lung model by spraying the surface with MTT was tested by killing bacteria with sterilizing UV radiation in a specific pattern (Fig. 7). The parts of the model shaded by aluminium foil (Fig. 7A) to protect them against UV-exposure exhibited purple-black colouration after spraying with MTT, indicating the existence of metabolically active bacteria. By contrast, the UV-exposed areas of the model where bacteria had been killed, remained colourless (Fig. 7B). Moreover, the versatility of the system was demonstrated by using various amounts of E. coli suspension incorporated in the agar layer (300 or 600 µl of suspension with OD600=0,2 per 20 ml LB-agar) and showing that the cell density correlated with the intensity of colour development after spraying with MTT solution (Fig. 8). This parameter can be optimized for individual bacterial test species.
Aerosol deposition and dose-dependency of bacterial inhibition with test antibiotics
Using an air-flow rate of 11 l/min and a 20 min flow time as the standard, the effect of increasing the concentration of allicin solution in the aerosol was tested. At 0.25 mM allicin, no growth inhibition of allicin-susceptible E. coli strain (E. coli MegaX DH10B™ T1R transformed with empty pRU1097 vector) was detected; however, at 0.5 mM allicin, a clear inhibition of bacterial growth on the bronchial surfaces at the carinal branch points was observed (Fig. 9). This presumably reflects the areas of maximum aerosol deposition and coincides with areas of locally high air-flow velocities (Fig. 6). With an aerosol of 1 mM allicin, the growth inhibition of allicin-susceptible E. coli strain was complete on all the bronchial surfaces throughout the model (Fig. 9). In total, 8 ml of the allicin test solution were passed through the model as an aerosol, meaning that the amounts of allicin aspirated through the model were 0.33, 0.65 and 1.2 mg, respectively. However, it still has to be investigated in further measurements what proportion of the aerosol was deposited on the surfaces and what proportion remained suspended in the transient air-flow.
The usefulness of the lung model in highlighting the differential resistance of bacteria to antibiotics is shown in Fig. 10. The dose-dependency of growth inhibition of the allicin-susceptible E. coli isolate (MegaX DH10B™ T1R) with the empty pRU1097 vector (30) was compared to bacteria made resistant to allicin by transforming E. coli with the recombinant pRU1097 vector containing an allicin-resistance-conferring genomic clone from an allicin-resistant Pseudomonas fluorescens strain isolated from garlic (29). The growth of the susceptible E. coli strain (empty vector control) was completely inhibited throughout the lung model by a 1 mM allicin aerosol, whereas the allicin-resistant genomic clone transformant exhibited only slight inhibition at the carinal surfaces of the first branch point. With an aerosol of 2 mM allicin, the inhibition of the resistant E. coli extended to the carinal surfaces of the 2nd and 3rd branch points and at 5 mM inhibition, it was complete over all the bronchial surfaces (Fig. 10A and B). This differential resistance was specific to allicin, as was shown by comparing the inhibition of the two E. coli strains to gentamicin. The pRU1097 vector carries gentamicin resistance as a selection marker and both the empty vector and the E. coli genomic clone transformants were equally susceptible to gentamicin aerosols over the 2–15 mM range (Fig. 10C). This observation, that the differential resistance of bacteria to antibiotics can be demonstrated in the simulated bronchial environment of the model, holds promise for the development of allicin, either alone or in combination with other antibiotics, for the treatment of actual lung diseases, including MDR strains.
Synergism between ethanol and allicin vapour
As allicin evaporates into the gas phase, an alternative to supplying it as an aerosol via the pulmonary route, would be to breathe in allicin molecules as a vapour. The efficacy of allicin vapour against allicin-susceptible E. coli was tested in the lung model. In these experiments, 2 ml of test solution with an exposed surface area of 5 cm2 had air passed over it through the lung model at 6 l/min. Neither the vapour from 42 mM allicin in aqueous solution, nor from 95% (v/v) ethanol caused any visible inhibition of E. coli growth following 40 min of treatment (Fig. 11A and B). The concentration and volume of the allicin solution remaining at the end of the experiment were measured and the values were used to calculate the amount of allicin, which had evaporated into the gas flow during the experiment. In the case of the 42 mM allicin solution, 1.27 mg of allicin had entered the gas phase and had passed through the model in a 40-min period. By contrast, a weaker 18.6 mM solution of allicin in 95% ethanol caused the inhibition of E. coli growth after only 20 min ‘inhalation’ (Fig. 11C). In the latter case, only 0.45 mg of allicin vapour had passed through the lung model. The degree of inhibition of E. coli growth increased progressively after 30 and 40 min of treatment with the vapour from the 18.6 mM ethanolic allicin solution, and by 40 min inhibition, was virtually complete (Fig. 11D and E). The 30- and 40-min ‘inhalations’ corresponded to 0.9 mg and maximally 6.0 mg, respectively, of allicin vapour passing through the lung model. There is some uncertainty as to the amount of allicin entering the vapour phase for the 40-min time point as under these conditions, the remainder of the 2 ml of solution had dried to a residue that could not be accurately quantified. The value of a maximum 6.0 mg would be correct if all the allicin in the original 2 ml solution had entered the vapour phase. Allicin has a high relative molecular mass (Mr=162.28) and is quite polar; thus, its vapour pressure (P) is relatively low; however, it is not easy to determine as it decomposes at the high temperatures needed to determine P experimentally. The results for the 20- and 30-min ‘inhalation’ periods clearly demonstrated a pronounced synergism between allicin and ethanol in the vapour phase in the inhibition of E. coli growth.
Discussion
In the case of lung infections, the delivery of antibiotics via the pulmonary rather than the oral route would seem advantageous for a number of reasons. Most importantly, the pulmonary route provides direct exposure of the antibiotic to the pathogen and does not rely on assimilation into the body and circulatory transport to achieve a therapeutic dose at the site of treatment. Moreover, for a substance, such as allicin from garlic, the oral route is in any case unrealistic as allicin will be very rapidly titrated out by glutathione with which it reacts readily to form S-allyl-mercapto-glutathione (6,7,14,31,32). Antibiotics can be delivered directly into the lungs either as an aerosol of droplets or as molecules in the gas phase as a vapour. Aerosols would be required for most of the conventional antibiotics as they are not volatile and do not enter the gas phase directly. Allicin, however is volatile, as attested to by the odour, it evaporates into the air by crushing or cutting garlic cloves. Nevertheless, due to its relatively large size and high polarity, its vapour pressure is not high. The efficacy of allicin aerosols and allicin vapour against E. coli were investigated in this study using the lung model. The ability of allicin and other thiosulfinates to inhibit bacteria, including lung pathogenic bacteria, as a vapour has been demonstrated in static Petri plate assays (11,31). The development of allicin as an antibiotic for clinical use is not completely straightforward, as although it can be easily synthesized (4), is not stable in solution for long periods of time unless it is frozen (33). Moreover, allicin is also inhibitory to mammalian cell growth in a dose-dependent manner (11,34). Furthermore, tissue burns from the inappropriate use of garlic preparations in attempts to treat various conditions have been well documented (2). Therefore, it remains to be determined whether there is a sufficient degree of differential toxicity between mammalian cells and the target microbes in each given situation to warrant the use of allicin as a treatment for infections. However, in this regard, it should be remembered that cells cultured in vitro are likely to be more sensitive than cells in vivo, where a continual supply of GSH in bodily fluids will help to protect them against allicin (11,31). For example, cells in the lung are continually supplied with blood and bathed with epithelial lining fluid (ELF) containing GSH in the mM range (35,36). Moreover, by virtue of their function in oxygen uptake, lung cells must be well protected against oxidative stress. With a substance, such as allicin which has an oxidative mechanism of action, GSH protects cells against the effects of allicin. Cells in culture, with only a fixed charge of GSH in the medium, will probably be more sensitive to allicin than cells in vivo which are continually bathed with new GSH which titrates out allicin. Bacteria colonizing the lung surface would be more immediately exposed to allicin than the underlying epithelial cells and this might be expected to contribute to a differential sensitivity of epithelial cells and bacteria the in vivo situation in lung disease (11). In this regard, the historical report of Minchin of the successful treatment of pulmonary tuberculosis with vapour from garlic pulp is significant (17). It should be noted that aerosols of allicin solutions could be potentially much more damaging to lung tissue than allicin vapour and this aspect must be carefully investigated before allicin can be used therapeutically. These speculations aside, at some point, the efficacy of allicin against lung pathogens must be tested in an animal model to address these unknowns directly. However, a number of potential treatment parameters can be determined in vitro using the lung test rig in pre-clinical trials and can thus reduce the number of animal experiments necessary.
A further difficulty for developing novel antibiotics is the emergence of resistance. Even though an antibiotic, such as allicin with multiple sites of action makes the appearance of spontaneous resistance in microbes difficult, it does not mean that it cannot occur, particularly under prolonged and severe selection pressure. Thus, high resistance against allicin has been found in some naturally occurring bacteria living in association with garlic and presumably facing a high selection pressure against allicin (29).
The test rig is scaled to cover the 2nd to 5th human bronchial passages. It has been reported that this is the region of the lung where pneumococcal pneumonia infections generally begin (37). Thus, using appropriate test bacteria, dosage regimes and aerosol deposition patterns which would be likely to be successful in treating developing lung infections could be determined before animal testing were undertaken. In this study, non-pathogenic E. coli MegaX DH10B™ T1R was used as a test bacterium to avoid the need for special containment requirements for working with human pathogens; however, it is envisaged that future tests with authentic pathogens will be carried out as necessary.
Using the lung model the dose-dependent efficacy of gentamicin and allicin aerosols against E. coli MegaX DH10B™ T1R was demonstrated (Figs. 9 and 10B and C). Furthermore, the model proved its potential for determining dosage regimes for bacteria exhibiting varying strengths of resistance to individual antibiotics (Fig. 10B and C). In these experiments, the E. coli strains used were isogenic apart from the 9 kb allicin resistance-conferring genomic clone from an allicin-resistant Pseudomonas fluorescens isolated from a garlic bulb (29). E. coli MegaX DH10B™ T1R transformants carrying this genomic clone exhibited increased allicin resistance, although both E. coli strains were equally susceptible to gentamicin. This situation is clearly recapitulated in the results of the aerosol dose-effectivity experiments shown in Fig. 10B and C, where it can clearly be seen that a larger dose of allicin was needed to achieve the same degree of inhibition of the resistant E. coli cells than the susceptible cells. By contrast, both strains responded equally to gentamicin. In this manner, treatment regimens tailored to individual resistance profiles of lung pathogenic bacteria might be optimized before progressing to animal trials.
Moreover, the potential of the model for highlighting synergism was demonstrated for ethanol and allicin in the gas phase (Fig. 11). Notably, the historical publication by Minchin which documents the use of garlic vapour to treat pulmonary tuberculosis in patient subjects, describes the preparation used for inhalation as ‘3½ ounces of succus allii sativi, 3½ drams sp. vini. and 1/2 dram of ol. eucalyp. Citriodora’ (17). In contemporary measurements, this would be 98 g of garlic pulp, 13 ml of ethanol and 1.85 ml of eucalyptus oil. Minchin states that the ethanol helped to mask the garlic odour and preserved the garlic pulp during storage, whereas the eucalyptus oil was solely to mask the odour of the garlic. It is tempting to speculate that the ethanol in Dr Minchin's preparations might have had an unsuspected synergistic effect with allicin, similar to the one observed with the lung model in this study, and enhanced the inhibition of the mycobacteria in the lungs of his test subjects. The use of the lung model to test for synergism between allicin and other antibiotics, or other combinations of antibiotics not including allicin, is a promising aspect of the model's potential use.
There is certainly scope for future improvement and further development of the basic lung model test rig so that it can more closely resemble a real lung. For example, at present, the test rig only allows a continual uni-directional air-flow and does not simulate actual breathing during which air-flow accelerates, then slows to a minimum and reverses direction as air is exhaled. Incorporating such a breathing simulation pattern will be very desirable in the future to more accurately model the exposure pattern of bronchial surfaces to test substances. Other improvements could be surface coatings allowing growth of lung epithelial cells (38), with a long term view to co-cultivation with pathogenic bacteria for a more realistic simulation of the disease situation. Ideally, such cultivated epithelial cells would be continually supplied with blood and bathed with epithelial lining fluid (ELF), containing GSH in the mM range (35,36). The technical difficulties which would need to be overcome for this near-natural situation will certainly not be trivial.
The present model could however, be easily adapted to be coated with growth medium which is seeded with fungal spores. With a suitable redox indicator, fungal growth inhibition by test substances could also be characterized using the test rig. Additionally, if test bacteria with a suitable bioluminescent reporter system were used to seed the agar coating the lung model surface, the quantitative response due to deposition of a test substance could be indicated in the different parts of the model and this would allow local deposition levels to be accurately determined. This information could also be used to design dosage parameters for any particular test substance as a prelude to animal experiments.
Acknowledgements
The authors would like to gratefully acknowledge Philipp Müller for Figs. 3, 4 and 6 and Ulrike Noll for the proofreading of the manuscript.
Funding
Financial support from the RWTH Aachen University (to JR, JB and AJS) is gratefully acknowledged. JR was supported by an RFwN Ph.D. stipendium.
Availability of data and materials
All data generated or analysed during this study are included in this published article or are available from the corresponding author on reasonable request.
Authors' contributions
JR, JB, PD, MK and contributed to the experimental work, data acquisition and analysis. AJS contributed to the conception and design of the experiments, data analysis and wrote the manuscript. MCHG and WS contributed to the conception of the study and critically revised the manuscript. All authors read and approved the final version of the manuscript.
Ethics approval and consent to participate
Not applicable.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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