Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells

Li,Xiaojun; Liu,Yibin; Liu,Qiang; Wang,Sa; Ma,Yang; Jin,Qunhua

doi:10.3892/etm.2020.8562

Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells

Authors:
- Xiaojun Li
- Yibin Liu
- Qiang Liu
- Sa Wang
- Yang Ma
- Qunhua Jin
View Affiliations

Affiliations: The Third Orthopedic Department, General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750001, P.R. China, General Clinical College, Ningxia Medical University, College of Medicine, Yinchuan, Ningxia 750004, P.R. China
Published online on: February 26, 2020 https://doi.org/10.3892/etm.2020.8562
Pages: 2879-2886
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Osteoarthritis (OA) is a degenerative joint disease that seriously affects the quality of life of patients. Irisin has been reported to regulate bone metabolism via the cellular autocrine mechanism and play a protective role in rat OA. In the present study, a SW1353 chondrosarcoma cell line was treated with interleukin (IL)‑1β and irisin. The present study evaluated cell viability, expression levels of collagen II (Col II) and matrix metalloproteinase‑13 (MMP‑13), and activity of the Wnt/β‑catenin and NF‑κB signaling pathways in treated SW1353 cells. The present results suggested that IL‑1β could decrease Col II expression and increase MMP‑13 expression at both the mRNA and protein levels, and also activate the Wnt/β‑catenin and NF‑κB signaling pathways in SW1353 cells. By contrast, irisin was identified to reverse the effects of IL‑1β in IL‑1β‑induced SW1353 cells. The present results suggested that irisin treatment may have a cartilage‑protective role in an IL‑1β‑induced SW1353 cell model.

View Figures

View References

1	Krasnokutsky S, Attur M, Palmer G, Samuels J and Abramson SB: Current concepts in the pathogenesis of osteoarthritis. Osteoarthritis Cartilage. 16 (Suppl 3):S1–S3. 2008.PubMed/NCBI View Article : Google Scholar
2	Buckland J: Osteoarthritis: Targeting cartilage erosion in OA. Nat Rev Rheumatol. 6(64)2010.PubMed/NCBI View Article : Google Scholar
3	Lahm A, Kasch R, Mrosek E, Spank H, Erggelet C, Esser J and Merk H: Semiquantitative analysis of ECM molecules in the different cartilage layers in early and advanced osteoarthritis of the knee joint. Histol Histopathol. 27:609–615. 2012.PubMed/NCBI View Article : Google Scholar
4	Wang JH, Shih KS, Wu YW, Wang AW and Yang CR: Histone deacetylase inhibitors increase microRNA-146a expression and enhance negative regulation of interleukin-1β signaling in osteoarthritis fbroblast-like synoviocytes. Osteoarthritis Cartilage. 21:1987–1996. 2013.PubMed/NCBI View Article : Google Scholar
5	Klatt AR, Klinger G, Paul-Klausch B, Kühn G, Renno JH, Wagener R, Paulsson M, Schmidt J, Malchau G and Wielckens K: Matrilin-3 activates the expression of osteoarthritis-associated genes in primary human chondrocytes. FEBS Lett. 583:3611–3617. 2009.PubMed/NCBI View Article : Google Scholar
6	Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Rasbach KA, Boström EA, Choi JH, Long JZ, et al: A PGC1-a-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 481:463–468. 2012.PubMed/NCBI View Article : Google Scholar
7	Faienza MF, Brunetti G, Sanesi L, Colaianni G, Celi M, Piacente L, D'Amato G, Schipani E, Colucci S and Grano M: High irisin levels are associated with better glycemic control and bone health in children with type 1 diabetes. Diab Res Clin Pract. 141:10–17. 2018.PubMed/NCBI View Article : Google Scholar
8	Colaianni G, Cuscito C, Mongelli T, Pignataro P, Buccoliero C, Liu P, Lu P, Sartini L, Di Comite M, Mori G, et al: The myokine irisin increases cortical bone mass. Proc Natl Acad Sci USA. 112:12157–12162. 2015.PubMed/NCBI View Article : Google Scholar
9	Zhang J, Valverde P, Zhu X, Murray D, Wu Y, Yu L, Jiang H, Dard MM, Huang J, Xu Z, et al: Exercise-induced irisin in bone and systemic irisin administration reveal new regulatory mechanisms of bone metabolism. Bone Res. 5(16056)2017.PubMed/NCBI View Article : Google Scholar
10	Weng X, Lin P, Liu F, et al: Achyranthes bidentata polysaccharides activate the Wnt/β-catenin signaling pathway to promote chondrocyte proliferation. Int J Mol Med. 3:1045–1050. 2014.PubMed/NCBI View Article : Google Scholar
11	Lotz MK and Carames B: Autophagy and cartilage homeostasis mechanisms in joint health, aging and OA. Nat Rev Rheumatol. 7:579–587. 2011.PubMed/NCBI View Article : Google Scholar
12	Liacini A, Sylvester J, Li WQ, Huang W, Dehnade F, Ahmad M and Zafarullah M: Induction of matrix metalloproteinase-13 gene expression by TNF-alpha is mediated by MAP kinases, AP-1, and NF-kappaB transcription factors in articular chondrocytes. Exp Cell Res. 288:208–217. 2003.PubMed/NCBI View Article : Google Scholar
13	Schaefer JF, Millham ML, de Crombrugghe B and Buckbinder L: FGF signaling antagonizes cytokine-mediated repression of Sox9 in SW1353 chondrosarcoma cells. Osteoarthritis Cartilage. 11:233–241. 2003.PubMed/NCBI View Article : Google Scholar
14	Huang Y, Wan G and Tao J: C1q/TNF-related protein-3 exerts the chondroprotective effects in IL-1β-treated SW1353 cells by regulating the FGFR1 signaling. Biomed Pharmacother. 85:41–46. 2017.PubMed/NCBI View Article : Google Scholar
15	Feng G and Zhang S: Salicin inhibits AGE-induced degradation of type II collagen and aggrecan in human SW1353 chondrocytes: Therapeutic potential in osteoarthritis. Artif Cells Nanomed Biotechnol. 47:1043–1049. 2019.PubMed/NCBI View Article : Google Scholar
16	Lu YC, Jayakumar T, Duann YF, Chou YC, Hsieh CY, Yu SY, Sheu JR and Hsiao G: Chondroprotective role of sesamol by inhibiting MMPs expression via retaining NF-κB signaling in activated SW1353 cells. J Agric Food Chem. 11:4969–4978. 2011.PubMed/NCBI View Article : Google Scholar
17	Tetsunaga T, Nishida K, Furumatsu T, Naruse K, Hirohata S, Yoshida A, Saito T and Ozaki T: Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells. Osteoarthritis Cartilage. 19:222–232. 2011.PubMed/NCBI View Article : Google Scholar
18	Cheng NT, Meng H, Ma LF, Zhang L, Yu HM, Wang ZZ and Guo A: Role of autophagy in the progression of osteoarthritis: The autophagy inhibitor, 3-methyladenine, aggravates the severity of experimental osteoarthritis. Int J Mol Med. 39:1224–1232. 2017.PubMed/NCBI View Article : Google Scholar
19	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar
20	Chen WP, Hu ZN, Jin LB and Wu LD: Licochalcone A inhibits MMPs and ADAMTSs via the NF-κB and Wnt/β-catenin signaling pathways in rat chondrocytes. Cell Physiol Biochem. 43:937–944. 2017.PubMed/NCBI View Article : Google Scholar
21	Troeberg L and Nagase H: Proteases involved in cartilage matrix degradation in osteoarthritis. Biochim Biophys Acta. 1824:133–145. 2012.PubMed/NCBI View Article : Google Scholar
22	Tchetverikov I, Lohmander LS, Verzijl N, Huizinga TW, TeKoppele JM, Hanemaaijer R and DeGroot J: MMP protein and activity levels in synovial fluid from patients with joint injury, inflammatory arthritis, and osteoarthritis. Ann Rheum Dis. 64:694–698. 2005.PubMed/NCBI View Article : Google Scholar
23	Tetlow LC, Adlam DJ and Woolley DE: Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: Associations with degenerative changes. Arthritis Rheum. 44:585–594. 2001.PubMed/NCBI View Article : Google Scholar
24	Matyas JR, Huang D, Chung M and Adams ME: Regional quantification of cartilage type II collagen and aggrecan messenger RNA in joints with early experimental osteoarthritis. Arthritis Rheum. 46:1536–1543. 2002.PubMed/NCBI View Article : Google Scholar
25	Colaianni G, Cuscito C, Mongelli T, Oranger A, Mori G, Brunetti G, Colucci S, Cinti S and Grano M: Irisin enhances osteoblast differentiation in vitro. Int J Endocrinol. 2014(902186)2014.PubMed/NCBI View Article : Google Scholar
26	Marcu KB, Otero M, Olivotto E, Borzi RM and Goldring MB: NF-кB signaling: Multiple angles to target OA. Curr Drug Targets. 11:599–613. 2010.PubMed/NCBI View Article : Google Scholar
27	MacDonald BT, Tamai K and He X: Wnt/β-catenin signaling: Components, mechanisms, and diseases. Dev Cell. 17:9–26. 2009.PubMed/NCBI View Article : Google Scholar
28	Roman-Blas JA and Jimenez SA: NF-kappaB as a potential therapeutic target in osteoarthritis and rheumatoid arthritis. Osteoarthritis Cartilage. 14:839–848. 2006.PubMed/NCBI View Article : Google Scholar
29	Ma B and Hottiger MO: Crosstalk between Wnt/β-catenin and NF-кB signaling pathway during inflammation. Front Immunol. 7(378)2016.PubMed/NCBI View Article : Google Scholar