MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5

Yue,Na; Ye,Ming; Zhang,Ran; Wang,Miao

doi:10.3892/etm.2020.8814

MicroRNA‑1307‑3p accelerates the progression of colorectal cancer via regulation of TUSC5

Authors:
- Na Yue
- Ming Ye
- Ran Zhang
- Miao Wang
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Affiliations: Department of Pathology, The Third Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), Urumqi, Xinjiang Uygur Autonomous Region 830011, P.R. China, Department of Pathology, Occupational Disease Hospital, Urumqi, Xinjiang Uygur Autonomous Region 830091, P.R. China
Published online on: May 28, 2020 https://doi.org/10.3892/etm.2020.8814
Pages: 1746-1751

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Abstract

The aim of the present study was to explore the roles of microRNA‑1307‑3p (miR‑1307‑3p) in colorectal cancer (CRC). Firstly, the expression level of miR‑1307‑3p in CRC cells was measured using reverse transcription‑quantitative PCR. Subsequently, Cell Counting Kit‑8 and Transwell invasion assays were performed to evaluate the effects of miR‑1307‑3p on CRC cell proliferation and invasion, respectively. Bioinformatics tools and dual luciferase reporter assays were used to validate the targets of miR‑1307‑3p. Rescue experiments were performed to confirm tumor suppressor candidate 5 (TUSC5) as a functional target of miR‑1307‑3p. miR‑1307‑3p levels were revealed to be upregulated in CRC cells when compared with the normal human epithelial cell line. Knockdown of miR‑1307‑3p inhibited CRC cell growth and invasiveness. Bioinformatics analysis and dual‑luciferase activity reporter assays demonstrated that miR‑1307‑3p binds the 3'‑untranslated region of TUSC5. Finally, rescue experiments validated that miR‑1307‑3p was able to regulate CRC cell behaviors via regulating TUSC5 expression. Together, the current results indicate that miR‑1307‑3p functions as an oncogenic miRNA via targeting TUSC5 in CRC.

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