Open Access

SNHG16 accelerates the proliferation of primary cardiomyocytes by targeting miRNA‑770‑5p

  • Authors:
    • Linshan Yang
    • Yan Lu
    • Jie Ming
    • Yuzhu Pan
    • Renbin Yu
    • Yuhui Wu
    • Tao Wang
  • View Affiliations

  • Published online on: July 29, 2020     https://doi.org/10.3892/etm.2020.9083
  • Pages: 3221-3227
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to clarify the influence of long non‑coding RNA small nuclear host gene 16 (lncRNA SNHG16) on cardiomyocyte proliferation following ischemia/reperfusion injury (IRI) and the potential mechanism. An IRI model in mice was established by performing ligation of the anterior descending coronary artery (LAD). Primary cardiomyocytes were isolated from newborn mice and subjected to H2O2 treatment to mimic in vitro IRI. Relative levels of SNHG16 and miRNA‑770‑5p in both in vivo and in vitro IRI models were examined. The regulatory effects of SNHG16 and miRNA‑770‑5p on the proliferative ability of H2O2‑treated cardiomyocytes were assessed by Cell Counting Kit‑8 (CCK‑8) and 5‑ethynyl‑2'‑deoxyuridine (EdU) assay. The binding relationship between SNHG16 and miRNA‑770‑5p was verified through dual‑luciferase reporter gene assay. It is found that SNHG16 was time‑dependently downregulated in the IRI models. Overexpression of SNHG16 enhanced the proliferative ability of the cardiomyocytes. miRNA‑770‑5p was found to be a direct target of SNHG16. Moreover, SNHG16 was able to negatively regulate the miRNA‑770‑5p level. Overexpression of miRNA‑770‑5p partially reversed the role of SNHG16 on accelerating cardiomyocyte proliferation. Collectively, SNHG16 accelerates the proliferative ability of cardiomyocytes following IRI by negatively regulating miRNA‑770‑5p.
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October-2020
Volume 20 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Yang L, Lu Y, Ming J, Pan Y, Yu R, Wu Y and Wang T: SNHG16 accelerates the proliferation of primary cardiomyocytes by targeting miRNA‑770‑5p. Exp Ther Med 20: 3221-3227, 2020.
APA
Yang, L., Lu, Y., Ming, J., Pan, Y., Yu, R., Wu, Y., & Wang, T. (2020). SNHG16 accelerates the proliferation of primary cardiomyocytes by targeting miRNA‑770‑5p. Experimental and Therapeutic Medicine, 20, 3221-3227. https://doi.org/10.3892/etm.2020.9083
MLA
Yang, L., Lu, Y., Ming, J., Pan, Y., Yu, R., Wu, Y., Wang, T."SNHG16 accelerates the proliferation of primary cardiomyocytes by targeting miRNA‑770‑5p". Experimental and Therapeutic Medicine 20.4 (2020): 3221-3227.
Chicago
Yang, L., Lu, Y., Ming, J., Pan, Y., Yu, R., Wu, Y., Wang, T."SNHG16 accelerates the proliferation of primary cardiomyocytes by targeting miRNA‑770‑5p". Experimental and Therapeutic Medicine 20, no. 4 (2020): 3221-3227. https://doi.org/10.3892/etm.2020.9083